RRC ID |
45349
|
著者 |
Sugimoto H, Watanabe K, Toyama T, Takahashi SS, Sugiyama S, Lee MC, Hamada N.
|
タイトル |
Inhibitory effects of French pine bark extract, Pycnogenol®, on alveolar bone resorption and on the osteoclast differentiation.
|
ジャーナル |
Phytother Res
|
Abstract |
Pycnogenol(®) (PYC) is a standardized bark extract from French maritime pine (Pinus pinaster Aiton). We examined the inhibitory effects of PYC on alveolar bone resorption, which is a characteristic feature of periodontitis, induced by Porphyromonas gingivalis (P. gingivalis) and osteoclast differentiation. In rat periodontitis model, rats were divided into four groups: group A served as the non-infected control, group B was infected orally with P. gingivalis ATCC 33277, group C was administered PYC in the diet (0.025%: w/w), and group D was infected with P. gingivalis and administered PYC. Administration of PYC along with P. gingivalis infection significantly reduced alveolar bone resorption. Treatment of P. gingivalis with 1 µg/ml PYC reduced the number of viable bacterial cells. Addition of PYC to epithelial cells inhibited adhesion and invasion by P. gingivalis. The effect of PYC on osteoclast formation was confirmed by tartrate-resistant acid phosphatase staining. PYC treatment significantly inhibited osteoclast formation. Addition of PYC (1-100 µg/ml) to purified osteoclasts culture induced cell apoptosis. These results suggest that PYC may prevent alveolar bone resorption through its antibacterial activity against P. gingivalis and by suppressing osteoclastogenesis. Therefore, PYC may be useful as a therapeutic and preventative agent for bone diseases such as periodontitis.
|
巻・号 |
29(2)
|
ページ |
251-9
|
公開日 |
2015-2-1
|
DOI |
10.1002/ptr.5245
|
PMID |
25336411
|
MeSH |
Acid Phosphatase
Alveolar Bone Loss / prevention & control*
Animals
Anti-Bacterial Agents / pharmacology
Bone and Bones / drug effects
Cell Differentiation / drug effects
Cell Line
Cells, Cultured
Epithelial Cells / drug effects
Flavonoids / pharmacology*
Gingiva / cytology
Humans
Isoenzymes
Male
Mice, Inbred BALB C
Osteoclasts / drug effects*
Periodontitis / microbiology
Periodontitis / prevention & control
Pinus / chemistry*
Plant Bark / chemistry
Plant Extracts
Porphyromonas gingivalis / drug effects
Rats, Sprague-Dawley
Tartrate-Resistant Acid Phosphatase
|
IF |
4.087
|
引用数 |
4
|
WOS 分野
|
CHEMISTRY, MEDICINAL
PHARMACOLOGY & PHARMACY
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-G2/PA6(RCB1127) |