| RRC ID |
45352
|
| 著者 |
Chibazakura T, Toriyabe Y, Fujii H, Takahashi K, Kawakami M, Kuwamura H, Haga H, Ogura S, Abe F, Nakajima M, Yoshikawa H, Tanaka T.
|
| タイトル |
5-Aminolevulinic acid enhances cell death under thermal stress in certain cancer cell lines.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
5-aminolevulinic acid (5-ALA) is contained in all organisms and a starting substrate for heme biosynthesis. Since administration of 5-ALA specifically leads cancer cells to accumulate protoporphyrin IX (PpIX), a potent photosensitizer, we tested if 5-ALA also serves as a thermosensitizer. 5-ALA enhanced heat-induced cell death of cancer cell lines such as HepG2, Caco-2, and Kato III, but not other cancer cell lines including U2-OS and normal cell lines including WI-38. Those 5-ALA-sensitive cancer cells, but neither U2-OS nor WI-38, accumulated intracellular PpIX and exhibited an increased reactive oxygen species (ROS) generation under thermal stress with 5-ALA treatment. In addition, blocking the PpIX-exporting transporter ABCG2 in U2-OS and WI-38 cells enhanced their cell death under thermal stress with 5-ALA. Finally, a ROS scavenger compromised the cell death enhancement by 5-ALA. These suggest that 5-ALA can sensitize certain cancer cells, but not normal cells, to thermal stress via accumulation of PpIX and increase of ROS generation.
|
| 巻・号 |
79(3)
|
| ページ |
422-31
|
| 公開日 |
2015-1-1
|
| DOI |
10.1080/09168451.2014.975186
|
| PMID |
25346276
|
| MeSH |
ATP Binding Cassette Transporter, Subfamily G, Member 2
ATP-Binding Cassette Transporters / metabolism
Aminolevulinic Acid / pharmacology*
Biological Transport / drug effects
Cell Death / drug effects
Cell Line, Tumor
Ferrochelatase / metabolism
Gene Expression Regulation, Neoplastic / drug effects
Heat-Shock Response / drug effects*
Humans
Intracellular Space / drug effects
Intracellular Space / metabolism
Iron-Binding Proteins / metabolism
Neoplasm Proteins / metabolism
Protoporphyrins / metabolism
Reactive Oxygen Species / metabolism
|
| IF |
1.516
|
| 引用数 |
4
|
|
WOS 分野
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
CHEMISTRY, APPLIED
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
MRC-5(RCB0218)
WI-38(RCB0702) |
