RRC ID 45358
Author Kudo H, Wada H, Sasaki H, Tsuji H, Otsuka R, Baghdadi M, Kojo S, Chikaraishi T, Seino K.
Title Induction of macrophage-like immunosuppressive cells from mouse ES cells that contribute to prolong allogeneic graft survival.
Journal PLoS One
Abstract Recent progress in regenerative medicine has enabled the utilization of pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs) as a donor resource for transplantation. However, immune suppression is still needed when the donor-recipient combination is allogeneic. Protection of ESCs-derived grafts from host immune response might be achieved thought the utilization of immunosuppressive cells generated from ESCs. In the present study, we show that a certain fraction of immunosuppressive cells can be generated from ESCs and help to suppress immune response against allogeneic grafts. ESCs-derived suppressor cells (ES-SCs) resembled macrophages in terms of cell surface molecule and gene expressions. Furthermore, gene expression analysis including microarray showed that ES-SCs have M1/M2 hybrid phenotype with high expression of genes correlated to immunosuppression of T cell response. Indeed, ES-SCs were effective to block allogeneic T cell proliferation in a nitric oxide-dependent manner, and prolonged the survival of ESCs-derived embryoid bodies or cardiomyocytes grafts transplanted into mouse kidney capsule. Thus, we consider the potential use of these ESCs-derived macrophage-like immunosuppressive cells as cellular therapies to promote long-term graft survival in future therapies.
Volume 9(10)
Pages e111826
Published 2014-1-1
DOI 10.1371/journal.pone.0111826
PII PONE-D-14-34143
PMID 25356669
PMC PMC4214817
MeSH Animals Cell Proliferation Dendritic Cells / cytology Female Graft Survival / immunology* Immunosuppression Therapy* Isoantigens / immunology Macrophages / cytology* Mice Mice, Inbred C3H Mouse Embryonic Stem Cells / cytology* Myocytes, Cardiac / cytology Myocytes, Cardiac / transplantation* Phenotype T-Lymphocytes / cytology Transplantation, Homologous
IF 2.74
Times Cited 7
Human and Animal Cells OP9(RCB1124)