| RRC ID |
45358
|
| 著者 |
Kudo H, Wada H, Sasaki H, Tsuji H, Otsuka R, Baghdadi M, Kojo S, Chikaraishi T, Seino K.
|
| タイトル |
Induction of macrophage-like immunosuppressive cells from mouse ES cells that contribute to prolong allogeneic graft survival.
|
| ジャーナル |
PLoS One
|
| Abstract |
Recent progress in regenerative medicine has enabled the utilization of pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs) as a donor resource for transplantation. However, immune suppression is still needed when the donor-recipient combination is allogeneic. Protection of ESCs-derived grafts from host immune response might be achieved thought the utilization of immunosuppressive cells generated from ESCs. In the present study, we show that a certain fraction of immunosuppressive cells can be generated from ESCs and help to suppress immune response against allogeneic grafts. ESCs-derived suppressor cells (ES-SCs) resembled macrophages in terms of cell surface molecule and gene expressions. Furthermore, gene expression analysis including microarray showed that ES-SCs have M1/M2 hybrid phenotype with high expression of genes correlated to immunosuppression of T cell response. Indeed, ES-SCs were effective to block allogeneic T cell proliferation in a nitric oxide-dependent manner, and prolonged the survival of ESCs-derived embryoid bodies or cardiomyocytes grafts transplanted into mouse kidney capsule. Thus, we consider the potential use of these ESCs-derived macrophage-like immunosuppressive cells as cellular therapies to promote long-term graft survival in future therapies.
|
| 巻・号 |
9(10)
|
| ページ |
e111826
|
| 公開日 |
2014-1-1
|
| DOI |
10.1371/journal.pone.0111826
|
| PII |
PONE-D-14-34143
|
| PMID |
25356669
|
| PMC |
PMC4214817
|
| MeSH |
Animals
Cell Proliferation
Dendritic Cells / cytology
Female
Graft Survival / immunology*
Immunosuppression Therapy*
Isoantigens / immunology
Macrophages / cytology*
Mice
Mice, Inbred C3H
Mouse Embryonic Stem Cells / cytology*
Myocytes, Cardiac / cytology
Myocytes, Cardiac / transplantation*
Phenotype
T-Lymphocytes / cytology
Transplantation, Homologous
|
| IF |
2.74
|
| 引用数 |
7
|
|
WOS 分野
|
IMMUNOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
OP9(RCB1124) |
