論文 - 詳細
| RRC ID | 45385 |
|---|---|
| 著者 | Miyatake Y, Sheehy N, Ikeshita S, Hall WW, Kasahara M. |
| タイトル | Anchorage-dependent multicellular aggregate formation induces CD44 high cancer stem cell-like ATL cells in an NF-κB- and vimentin-dependent manner. |
| ジャーナル | Cancer Lett |
| Abstract |
Adult T-cell leukemia/lymphoma (ATL) is an intractable T-cell malignancy accompanied by massive invasion of lymphoma cells into various tissues. We demonstrate here that ATL cells cultured on a layer of epithelial-like feeder cells form anchorage-dependent multicellular aggregates (Ad-MCAs) and that a fraction of MCA-forming ATL cells acquire CD44 high cancer stem cell-like phenotypes. ATL cells forming Ad-MCAs displayed extracellular microvesicles with enhanced expression of CD44v9 at cell synapses, augmented expression of multidrug resistance protein 1, and increased NF-κB activity. Blockade of the NF-κB pathway dramatically reduced Ad-MCA formation by ATL cells and the emergence of CD44 high ATL cells, but left a considerable number of ATL cells adhering to the feeder layer. Disruption of vimentin cytoskeleton by treatment with withaferin A, a natural steroidal lactone, suppressed not only the adhesion of ATL cells to the feeder layer but also subsequent Ad-MCA formation by ATL cells, suggesting the involvement of vimentin in anchoring ATL cells to the feeder layer. Ad-MCA formation by ATL cells on a layer of epithelial-like feeder cells may mimic critical events that occur in metastatic colonization. |
| 巻・号 | 357(1) |
| ページ | 355-363 |
| 公開日 | 2015-2-1 |
| DOI | 10.1016/j.canlet.2014.11.055 |
| PII | S0304-3835(14)00740-X |
| PMID | 25448402 |
| MeSH | Apoptosis / physiology HEK293 Cells Humans Hyaluronan Receptors / metabolism* Jurkat Cells Leukemia-Lymphoma, Adult T-Cell / metabolism* Leukemia-Lymphoma, Adult T-Cell / pathology* NF-kappa B / metabolism* Neoplastic Stem Cells / metabolism* Neoplastic Stem Cells / pathology Tumor Cells, Cultured Vimentin / metabolism* |
| IF | 7.36 |
| 引用数 | 10 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 1C3IKEI(RCB0797) |