| RRC ID |
45386
|
| 著者 |
Nakamura H, Murakami T, Hattori S, Sakaki Y, Ohkuri T, Chijiwa T, Ohno M, Oda-Ueda N.
|
| タイトル |
Epithelium specific ETS transcription factor, ESE-3, of Protobothrops flavoviridis snake venom gland transactivates the promoters of venom phospholipase A2 isozyme genes.
|
| ジャーナル |
Toxicon
|
| Abstract |
Protobothrops flavoviridis (habu) (Crotalinae, Viperidae) is a Japanese venomous snake, and its venom contains the enzymes with a variety of physiological activities. The phospholipases A2 (PLA2s) are the major components and exert various toxic effects. They are expressed abundantly in the venom gland. It is thought that the venom gland-specific transcription factors play a key role for activation of PLA2 genes specifically expressed in the venom gland. Thus, the full-length cDNA library for P. flavoviridis venom gland after milking of the venom was made to explore the transcription factors therein. As a result, three cDNAs encoding epithelium-specific ETS transcription factors (ESE)-1, -2, and -3 were obtained. Among them, ESE-3 was specifically expressed in the venom gland and activated the proximal promoters of venom PLA2 genes, which are possibly regarded as the representatives of the venom gland-specific protein genes in P. flavoviridis. Interestingly, the binding specificity of ESE-3 to the ETS binding motif located near TATA box is well correlated with transcriptional activities for the venom PLA2 genes. This is the first report that venom gland-specific transcription factor could actually activate the promoters of the venom protein genes.
|
| 巻・号 |
92
|
| ページ |
133-9
|
| 公開日 |
2014-12-15
|
| DOI |
10.1016/j.toxicon.2014.10.001
|
| PII |
S0041-0101(14)00332-8
|
| PMID |
25449102
|
| MeSH |
Animals
Base Sequence
CHO Cells
Cricetinae
Cricetulus
Crotalid Venoms / chemistry*
DNA Primers / genetics
DNA, Complementary / genetics
Electrophoretic Mobility Shift Assay
Enzyme Activation / genetics*
Exocrine Glands / metabolism
Expressed Sequence Tags
Fluorescein-5-isothiocyanate
Genetic Vectors / genetics
Isoenzymes / genetics
Japan
Luciferases
Molecular Sequence Data
Phospholipases A2 / genetics*
Phospholipases A2 / metabolism
Promoter Regions, Genetic / genetics
Proto-Oncogene Proteins c-ets / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Viperidae / metabolism*
|
| IF |
2.201
|
| 引用数 |
2
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
TOXICOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
CHO-K1(RCB0285) |
