Reference - Detail
| RRC ID | 45417 |
|---|---|
| Author | Nagase K, Hatakeyama Y, Shimizu T, Matsuura K, Yamato M, Takeda N, Okano T. |
| Title | Thermoresponsive cationic copolymer brushes for mesenchymal stem cell separation. |
| Journal | Biomacromolecules |
| Abstract |
Thermoresponsive, cationic, copolymer brushes poly(N-isopropylacrylamide(IPAAm)-co-N,N-dimethylaminopropylacrylamide-co-N-tert-butylacrylamide(tBAAm)) and poly(IPAAm-co-3-acrylamidopropyl trimethylammonium chloride-co-tBAAm) were prepared on glass substrates through surface-initiated atom transfer radical polymerization. Prepared copolymer brushes were investigated as thermally modulated cell separation materials. Densely packed cationic copolymer brushes were formed on the glass substrates, and the positive charge density was modulated by controlling the composition of cationic moieties and species. During observation of cell adhesion and detachment properties on copolymer brushes, human bone marrow mesenchymal stem cells (hbmMSC) exhibited thermally modulated cell adhesion and detachment, while other bone-marrow-derived cells did not adhere. Using these properties, hbmMSC could be purified from mixtures of human bone-marrow-derived cells simply by changing the external temperature. Therefore, the prepared cationic copolymer brush is useful for separation of hbmMSC. |
| Volume | 16(2) |
| Pages | 532-40 |
| Published | 2015-2-9 |
| DOI | 10.1021/bm501591s |
| PMID | 25517012 |
| MeSH | Cell Adhesion / drug effects Cell Adhesion / physiology Cell Separation / methods* Cells, Cultured Humans Mesenchymal Stem Cells / cytology* Mesenchymal Stem Cells / drug effects Polymerization Polymers / chemistry* Polymers / pharmacology Temperature* |
| IF | 6.092 |
| Times Cited | 33 |
| WOS Category | CHEMISTRY, ORGANIC POLYMER SCIENCE BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
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| The most frequently cited source | |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Human mesenchymal stem cells |