論文 - 詳細
| RRC ID | 45418 |
|---|---|
| 著者 | Satoh T, Igarashi M, Yamada S, Takahashi N, Watanabe K. |
| タイトル | Inhibitory effect of black tea and its combination with acarbose on small intestinal α-glucosidase activity. |
| ジャーナル | J Ethnopharmacol |
| Abstract |
ETHNOPHARMACOLOGICAL RELEVANCE:It is said that black tea is effective against type 2 diabetes mellitus because it can help modulate postprandial hyperglycemia. However, the mechanism underlying its therapeutic and preventive effects on type 2 diabetes mellitus is unclear. In this study, we focused on the effect of black tea on the carbohydrate digestion and absorption process in the gastrointestinal tract. We examined whether black tea can modulate postprandial hyperglycemia. MATERIALS AND METHODS:The freeze-dried powder of the aqueous extract of black tea leaves (JAT) was used for in vitro studies of α-amylase activity, α-glucosidase activity, and glucose uptake by glucose transporters in Caco-2 cells; ex vivo studies of small intestinal α-glucosidase activity; and in vivo studies of oral sugar tolerance in GK rats, an animal model of nonobese type 2 diabetes mellitus. RESULTS:Half maximal inhibitory concentration values indicated that JAT significantly reduced α-glucosidase activity, but weakly reduced α-amylase activity. Kinetic studies of rat small intestinal α-glucosidase activity revealed that the combination of JAT and the α-glucosidase inhibitor, acarbose, showed a mixed-type inhibition. JAT had no effect on the uptake of 2'-deoxy-d-glucose by glucose transporter 2 (GLUT2) and the uptake of α-methyl-d-glucose by sodium-dependent glucose transporter 1 (SGLT1). In the oral sucrose tolerance test in GK rats, JAT reduced plasma glucose levels in a dose-dependent manner compared with the control group. The hypoglycemic action of JAT was also confirmed: JAT, in combination with acarbose, produced a synergistic inhibitory effect on plasma glucose levels in vivo. In contrast to the oral sucrose tolerance test, JAT showed no effect in the oral glucose tolerance test. CONCLUSIONS:JAT was demonstrated to inhibit the degradation of disaccharides into monosaccharides by α-glucosidase in the small intestine. Thereby indirectly preventing the absorption of the dietary source of glucose mediated by SGLT1 and GLUT2 transporters localized at the apical side of enterocytes in the small intestine. The results indicate that black tea could be useful as a functional food in the dietary therapy for borderline type 2 diabetes mellitus that could modulate postprandial hyperglycemia. |
| 巻・号 | 161 |
| ページ | 147-55 |
| 公開日 | 2015-2-23 |
| DOI | 10.1016/j.jep.2014.12.009 |
| PII | S0378-8741(14)00880-0 |
| PMID | 25523370 |
| MeSH | Acarbose / pharmacology* Animals Biflavonoids / analysis Blood Glucose / analysis Caco-2 Cells Caffeine / analysis Camellia sinensis* Catechin / analysis Drug Synergism Glucose / metabolism Glucose Transporter Type 2 / metabolism Glycoside Hydrolase Inhibitors / pharmacology* Humans Hyperglycemia / diet therapy Hyperglycemia / metabolism Intestine, Small / drug effects* Intestine, Small / metabolism Male Plant Extracts / chemistry Plant Extracts / pharmacology* Plant Extracts / therapeutic use Plant Leaves Polysaccharides / analysis Rats Sodium-Glucose Transporter 1 / metabolism alpha-Amylases / antagonists & inhibitors alpha-Amylases / metabolism alpha-Glucosidases / metabolism |
| IF | 3.69 |
| 引用数 | 51 |
| WOS 分野 | INTEGRATIVE & COMPLEMENTARY MEDICINE PHARMACOLOGY & PHARMACY CHEMISTRY, MEDICINAL PLANT SCIENCES |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |