RRC ID 45423
著者 Manaka S, Tanabe N, Kariya T, Naito M, Takayama T, Nagao M, Liu D, Ito K, Maeno M, Suzuki N, Miyazaki M.
タイトル Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells.
ジャーナル FEBS Lett
Abstract Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.
巻・号 589(3)
ページ 310-8
公開日 2015-1-30
DOI 10.1016/j.febslet.2014.12.013
PII S0014-5793(14)00893-X
PMID 25542352
MeSH Adenosine Triphosphate / genetics Adenosine Triphosphate / metabolism Alkaline Phosphatase / metabolism Animals Cell Differentiation / genetics* Cell Differentiation / radiation effects Cell Proliferation / radiation effects Humans Mice Osteoblasts / metabolism Osteoblasts / radiation effects Osteogenesis / genetics* Osteogenesis / radiation effects Receptors, Purinergic P2X7 / genetics Receptors, Purinergic P2X7 / metabolism* Sound*
IF 3.057
引用数 24
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)