RRC ID 45428
Author Yamada Y, Perez SM, Tabata M, Abe J, Yasuzaki Y, Harashima H.
Title Efficient and High-Speed Transduction of an Antibody into Living Cells Using a Multifunctional Nanocarrier System to Control Intracellular Trafficking.
Journal J Pharm Sci
Abstract The transduction of antibodies into living cells would represent a major contribution to both basic and applied biomedical fields, as currently available methods suffer from limitations such as low-uptake efficiency and endosomal entrapment. In this study, a liposome-based carrier was designed to overcome these issues. Liposomes were modified with octaarginine (R8), a cell penetrating peptide and GALA, a pH-sensitive fusogenic peptide. The presence of R8 enhanced the cellular uptake of antibodies, whereas GALA reduced endosomal entrapment, resulting in antibodies being released into the cytosol within 30 min. Moreover, compared with commercially available reagents for delivering antibodies, our system was superior in both cellular uptake and endosomal escape. In addition, specific antibodies delivered by R8-GALA liposomes were found to be associated with their epitope, confirming the preservation of functionality. This system for the efficient and high-speed cytosolic delivery of an antibody provides a valuable tool that can be useful in basic and applied research for analyzing the expression and function of intracellular molecules.
Volume 104(9)
Pages 2845-54
Published 2015-9-1
DOI 10.1002/jps.24310
PII S0022-3549(16)30064-8
PMID 25546552
MeSH Antibodies / metabolism* Antibody Specificity Cytosol / metabolism Drug Carriers / pharmacology* Drug Delivery Systems Endosomes / metabolism HeLa Cells Humans Liposomes Nanostructures* Oligopeptides / chemistry Peptides / chemistry
IF 2.997
Times Cited 9
Human and Animal Cells HeLa