論文 - 詳細
| RRC ID | 45437 |
|---|---|
| 著者 | Usui K, Ikeda T, Horibe Y, Nakao M, Hoshino T, Mizushima T. |
| タイトル | Identification of HSP70-inducing activity in Arnica montana extract and purification and characterization of HSP70-inducers. |
| ジャーナル | J Dermatol Sci |
| Abstract |
BACKGROUND:The expression of heat shock proteins (HSPs), particularly HSP70, is receiving considerable attention in the field of cosmetics, particularly given our recent report that ultraviolet-induced melanin production, skin damage and wrinkle formation were all suppressed in transgenic mice expressing HSP70. OBJECTIVE:In the present study, we searched for HSP70-inducers from a library of herbal extracts that have already been approved as quasi-pharmaceutical products in Japan. We selected an ethanol extract of Arnica montana (A. montana), based on its high HSP70-inducing activity and low cytotoxicity. METHODS:Cell viability was determined by MTT method and expression of HPS70 was monitored by immunoblotting analysis. RESULTS:From the extract, we purified and identified eight sesquiterpene lactones (AM1-8) as HSP70-inducers, among which AM-2 (helenalin 2-methylbutyrate) was selected due to its good HSP70-inducing properties and low cytotoxicity. Treatment of cultured mouse melanoma cells with AM-2 or A. montana extract up-regulated the expression of HSP70 in a dose-dependent manner. This treatment also activated heat shock factor-1, a transcription factor for hsp genes. Furthermore, pre-treatment of cells with AM-2 or A. montana extract decreased melanin production and expression and activity of tyrosinase. CONCLUSION:These results suggest that AM-2 and A. montana extract could be beneficial for use in hypopigmenting cosmetics as a consequence of their stimulatory effects on HSP70 expression. |
| 巻・号 | 78(1) |
| ページ | 67-75 |
| 公開日 | 2015-4-1 |
| DOI | 10.1016/j.jdermsci.2015.01.014 |
| PII | S0923-1811(15)00039-0 |
| PMID | 25724362 |
| MeSH | Animals Arnica* / chemistry Cell Line, Tumor Cell Survival / drug effects Dose-Response Relationship, Drug Ethanol / chemistry HSP70 Heat-Shock Proteins / metabolism* Melanins / biosynthesis Melanoma, Experimental Mice Monophenol Monooxygenase / metabolism Phytotherapy Plant Extracts / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology* Plants, Medicinal Skin / drug effects* Skin / metabolism Skin / pathology Skin Lightening Preparations / chemistry Skin Lightening Preparations / isolation & purification Skin Lightening Preparations / pharmacology* Skin Pigmentation / drug effects* Solvents / chemistry Up-Regulation |
| IF | 3.681 |
| 引用数 | 7 |
| WOS 分野 | DERMATOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | B16 melanoma 4A5(RCB0557) |