RRC ID 45443
Author Onishi R, Ohnishi S, Higashi R, Watari M, Yamahara K, Okubo N, Nakagawa K, Katsurada T, Suda G, Natsuizaka M, Takeda H, Sakamoto N.
Title Human Amnion-Derived Mesenchymal Stem Cell Transplantation Ameliorates Dextran Sulfate Sodium-Induced Severe Colitis in Rats.
Journal Cell Transplant
Abstract Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine. Recently, several studies have shown that MSCs can be easily isolated from human amnion. In this study, we investigated the therapeutic effect of human amnion-derived MSCs (AMSCs) in rats with severe colitis. Colitis was induced by the administration of 8% dextran sulfate sodium (DSS) from day 0 to day 5, and AMSCs (1 × 10(6) cells) were transplanted intravenously on day 1. Rats were sacrificed on day 5, and the colon length and histological colitis score were evaluated. The extent of inflammation was evaluated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and immunohistochemistry. The effect of AMSCs on the inflammatory signals was investigated in vitro. AMSC transplantation significantly ameliorated the disease activity index score, weight loss, colon shortening, and the histological colitis score. mRNA expression levels of proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and migration inhibitory factor (MIF) were significantly decreased in the rectums of AMSC-treated rats. In addition, the infiltration of monocytes/macrophages was significantly decreased in AMSC-treated rats. In vitro experiments demonstrated that activation of proinflammatory signals induced by TNF-α or lipopolysaccharide (LPS) in immortalized murine macrophage cells (RAW264.7) was significantly attenuated by coculturing with AMSCs or by culturing with a conditioned medium obtained from AMSCs. Although the phosphorylation of IκB induced by TNF-α or LPS was not inhibited by the conditioned medium, nuclear translocation of NF-κB was significantly inhibited by the conditioned medium. Taken together, AMSC transplantation provided significant improvement in rats with severe colitis, possibly through the inhibition of monocyte/macrophage activity and through inhibition of NF-κB activation. AMSCs could be considered as a new cell source for the treatment of severe colitis.
Volume 24(12)
Pages 2601-14
Published 2015-1-1
DOI 10.3727/096368915X687570
PII content-CT-1207_Onishi_et_al
PMID 25812083
MeSH Active Transport, Cell Nucleus / drug effects Adipocytes / cytology Adipogenesis / physiology Amnion / cytology* Animals Cell- and Tissue-Based Therapy / methods* Cells, Cultured Coculture Techniques Colitis / chemically induced Colitis / therapy* Culture Media, Conditioned / pharmacology Dextran Sulfate Humans I-kappa B Proteins / metabolism Immunohistochemistry Inflammation / therapy Interleukin-1beta / biosynthesis Interleukin-1beta / genetics Intramolecular Oxidoreductases / biosynthesis Intramolecular Oxidoreductases / genetics Lipopolysaccharides Macrophage Migration-Inhibitory Factors / biosynthesis Macrophage Migration-Inhibitory Factors / genetics Macrophages / immunology Male Mesenchymal Stem Cell Transplantation* Mesenchymal Stem Cells / physiology* Osteocytes / cytology Osteogenesis / physiology Phosphorylation RNA, Messenger / biosynthesis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Tumor Necrosis Factor-alpha / biosynthesis Tumor Necrosis Factor-alpha / genetics
IF 3.341
Times Cited 27
Human and Animal Cells 293(RCB1637)