論文 - 詳細
| RRC ID | 45448 |
|---|---|
| 著者 | Fujita T, Yuno M, Okuzaki D, Ohki R, Fujii H. |
| タイトル | Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing. |
| ジャーナル | PLoS One |
| Abstract |
Accumulating evidence suggests that RNAs interacting with genomic regions play important roles in the regulation of genome functions, including X chromosome inactivation and gene expression. However, to our knowledge, no non-biased methods of identifying RNAs that interact with a specific genomic region have been reported. Here, we used enChIP-RNA-Seq, a combination of engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) and RNA sequencing (RNA-Seq), to perform a non-biased search for RNAs interacting with telomeres. In enChIP-RNA-Seq, the target genomic regions are captured using an engineered DNA-binding molecule such as a transcription activator-like protein. Subsequently, RNAs that interact with the target genomic regions are purified and sequenced. The RNAs detected by enChIP-RNA-Seq contained known telomere-binding RNAs, including the telomerase RNA component (Terc), the RNA component of mitochondrial RNA processing endoribonuclease (Rmrp), and Cajal body-specific RNAs. In addition, a number of novel telomere-binding non-coding RNAs were also identified. Binding of two candidate non-coding RNAs to telomeres was confirmed by immunofluorescence microscopy and RNA fluorescence in situ hybridization (RNA-FISH) analyses. The novel telomere-binding non-coding RNAs identified here may play important roles in telomere functions. To our knowledge, this study is the first non-biased identification of RNAs associated with specific genomic regions. The results presented here suggest that enChIP-RNA-Seq analyses are useful for the identification of RNAs interacting with specific genomic regions, and may help to contribute to current understanding of the regulation of genome functions. |
| 巻・号 | 10(4) |
| ページ | e0123387 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0123387 |
| PII | PONE-D-14-40469 |
| PMID | 25874893 |
| PMC | PMC4395285 |
| MeSH | Animals Base Sequence Cell Line Cell Line, Tumor Chromatin Immunoprecipitation* Genetic Engineering Humans In Situ Hybridization, Fluorescence Mice Molecular Sequence Data RNA / genetics RNA, Untranslated / genetics* Sequence Analysis, RNA* Telomerase / genetics Telomere / ultrastructure* |
| IF | 2.74 |
| 引用数 | 13 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB0805) |