| RRC ID |
45462
|
| 著者 |
Arahara K, Matsumoto T, Morimatsu F, Arai K.
|
| タイトル |
Fibronectin modified expression of Sonic hedgehog in ATRA-mediated neuronal differentiation.
|
| ジャーナル |
J Vet Med Sci
|
| Abstract |
In this study, the effect of fibronectin on the neurite outgrowth from embryoid bodies (EBs) in neurodifferentiated embryonal carcinoma P19 cells was examined. The neurite outgrowth on fibronectin was maintained for a longer time in comparison with those on collagen or laminin. Quantitative RT-PCR revealed that mRNA level corresponding to sonic hedgehog (Shh) in neurodifferentiated P19 cells was upregulated on fibronectin, whereas collagen or laminin did not affect. Further knockdown of integrin αv subunit in P19 cells demonstrated that expression of Shh was mediated through interaction between fibronectin and integrin. Additionally, exogenous Shh agonist accelerated neurite outgrowth from embryonic stem cell-derived EBs without large change of neuronal phenotype expression. Taken together, fibronectin could maintain neurite outgrowth via increased Shh expression.
|
| 巻・号 |
77(11)
|
| ページ |
1503-6
|
| 公開日 |
2015-11-1
|
| DOI |
10.1292/jvms.15-0122
|
| PMID |
26051001
|
| PMC |
PMC4667672
|
| MeSH |
Animals
Cell Differentiation
Cell Line, Tumor
Fibronectins / pharmacology*
Gene Expression Regulation / drug effects*
Hedgehog Proteins / genetics
Hedgehog Proteins / metabolism*
Mice
Neurons / physiology*
RNA, Messenger / genetics
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tretinoin / metabolism*
Up-Regulation
|
| IF |
1.049
|
| 引用数 |
2
|
|
WOS 分野
|
VETERINARY SCIENCES
|
| リソース情報 |
| ヒト・動物細胞 |
EB3(AES0139) |
