論文 - 詳細
| RRC ID | 45466 |
|---|---|
| 著者 | Maruyama K, Haniu H, Saito N, Matsuda Y, Tsukahara T, Kobayashi S, Tanaka M, Aoki K, Takanashi S, Okamoto M, Kato H. |
| タイトル | Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells. |
| ジャーナル | Biomed Res Int |
| Abstract |
Bronchial epithelial cells and mesothelial cells are crucial targets for the safety assessment of inhalation of carbon nanotubes (CNTs), which resemble asbestos particles in shape. Intrinsic properties of multiwalled CNTs (MWCNTs) are known to cause potentially hazardous effects on intracellular and extracellular pathways. These interactions alter cellular signaling and affect major cell functions, resulting in cell death, lysosome injury, reactive oxygen species production, apoptosis, and cytokine release. Furthermore, CNTs are emerging as a novel class of autophagy inducers. Thus, in this study, we focused on the mechanisms of MWCNT uptake into the human bronchial epithelial cells (HBECs) and human mesothelial cells (HMCs). We verified that MWCNTs are actively internalized into HBECs and HMCs and were accumulated in the lysosomes of the cells after 24-hour treatment. Next, we determined which endocytosis pathways (clathrin-mediated, caveolae-mediated, and macropinocytosis) were associated with MWCNT internalization by using corresponding endocytosis inhibitors, in two nonphagocytic cell lines derived from bronchial epithelial cells and mesothelioma cells. Clathrin-mediated endocytosis inhibitors significantly suppressed MWCNT uptake, whereas caveolae-mediated endocytosis and macropinocytosis were also found to be involved in MWCNT uptake. Thus, MWCNTs were positively taken up by nonphagocytic cells, and their cytotoxicity was closely related to these three endocytosis pathways. |
| 巻・号 | 2015 |
| ページ | 793186 |
| 公開日 | 2015-1-1 |
| DOI | 10.1155/2015/793186 |
| PMID | 26090445 |
| PMC | PMC4450259 |
| MeSH | Apoptosis / drug effects Asbestos / toxicity Autophagy / drug effects Bronchi / drug effects Bronchi / pathology Endocytosis / drug effects* Epithelial Cells / drug effects Epithelial Cells / pathology* Humans Mesothelioma / chemically induced Mesothelioma / pathology* Nanotubes, Carbon / adverse effects* Reactive Oxygen Species / metabolism |
| IF | 2.276 |
| 引用数 | 29 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ACC-MESO-1(RCB2292) |