RRC ID 45471
Author Kambe Y, Kojima K, Tamada Y, Tomita N, Kameda T.
Title Silk fibroin sponges with cell growth-promoting activity induced by genetically fused basic fibroblast growth factor.
Journal J Biomed Mater Res A
Abstract Transgenic silkworm technology has enabled the biological properties of silk fibroin protein to be altered by fusion to recombinant bioactive proteins. However, few studies have reported the fabrication of genetically modified fibroin proteins into three-dimensional spongy structures to serve as scaffolds for tissue engineering. We generated a transgenic silkworm strain that produces fibroin fused to basic fibroblast growth factor (bFGF) and processed the fibroin into a spongy structure using a simple freeze/thaw method. NIH3T3 mouse embryonic fibroblasts grown on bFGF-fused fibroin sponges proliferated and spread out well, showing half the population doubling time of cells cultured on wild-type fibroin sponges. Furthermore, the number of primary rabbit articular chondrocytes growing on bFGF-fused fibroin sponges was around five-times higher than that of the wild-type control at 3-days post cell-seeding. As the physical properties of wild-type and bFGF-fused fibroin sponges were almost identical, it is suggested that bFGF fused to fibroin retained its biological activity, even after the bFGF-fused fibroin was fabricated into the spongy structure. The bFGF-fused fibroin sponge has the potential for widespread application in the field of tissue engineering, and the method of fabricating this structure could be applicable to other recombinant bioactive fibroin proteins.
Volume 104(1)
Pages 82-93
Published 2016-1-1
DOI 10.1002/jbm.a.35543
PMID 26190702
MeSH Animals Blotting, Western Bombyx Cartilage, Articular / cytology Cell Proliferation / drug effects Cells, Cultured Chondrocytes / cytology* Chondrocytes / drug effects Electrophoresis, Polyacrylamide Gel Fibroblast Growth Factor 2 / pharmacology* Fibroblasts / cytology* Fibroblasts / drug effects Fibroins / pharmacology* Genetic Engineering* Genetic Vectors / metabolism Humans Mice NIH 3T3 Cells Porifera / chemistry* Rabbits
IF 3.221
Times Cited 13
Human and Animal Cells NIH3T3