論文 - 詳細
| RRC ID | 45474 |
|---|---|
| 著者 | Tahara Y, Yasuoka J, Sawada S, Sasaki Y, Akiyoshi K. |
| タイトル | Effective CpG DNA delivery using amphiphilic cycloamylose nanogels. |
| ジャーナル | Biomater Sci |
| Abstract |
Unmethylated CpG oligodeoxynucleotides induce inflammatory immune responses through cytokine production and have attracted increasing attention as an immunostimulator. However, there remains a challenging issue of the use of 'native CpG DNA'. In the present study, we prepared cationic nanometer-sized gels (nanogels) consisting of cycloamylose modified with cholesterol and diethylaminoethane to form hydrophobic cross-linking points and to add positively charged groups, respectively. The cationic nanogels and native CpG DNA formed nanometer-sized complexes. Complexes of native CpG DNA with cationic nanogels delivered native CpG DNA to macrophage-like cells and induced cytokine production. In addition, complexes of negative control oligonucleotides with cationic nanogels did not induce cytokine production, and the induction of cytokines using complexes of phosphorothioate-modified CpG with cationic nanogels was lower than that of native CpG DNA. These results suggest that the complex of native CpG DNA with cationic nanogels is a promising strategy for nucleic acid adjuvants. |
| 巻・号 | 3(2) |
| ページ | 256-64 |
| 公開日 | 2015-2-1 |
| DOI | 10.1039/c4bm00293h |
| PMID | 26218116 |
| MeSH | Cations Cholesterol / chemistry* Cyclodextrins / chemistry* Cyclodextrins / metabolism Cytokines / chemistry* Cytokines / metabolism DNA / administration & dosage DNA / chemistry* Drug Carriers Ethylamines / chemistry Liposomes / chemistry* Liposomes / metabolism Macrophages / chemistry* Macrophages / drug effects Macrophages / metabolism* Nanogels Oligodeoxyribonucleotides Phosphorothioate Oligonucleotides / chemistry* Phosphorothioate Oligonucleotides / metabolism Polyethylene Glycols / chemistry* Polyethyleneimine / chemistry* |
| IF | 6.183 |
| 引用数 | 10 |
| WOS 分野 | MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |