| RRC ID |
45495
|
| Author |
Konagaya S, Ando T, Yamauchi T, Suemori H, Iwata H.
|
| Title |
Long-term maintenance of human induced pluripotent stem cells by automated cell culture system.
|
| Journal |
Sci Rep
|
| Abstract |
Pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem (iPS) cells, are regarded as new sources for cell replacement therapy. These cells can unlimitedly expand under undifferentiated conditions and be differentiated into multiple cell types. Automated culture systems enable the large-scale production of cells. In addition to reducing the time and effort of researchers, an automated culture system improves the reproducibility of cell cultures. In the present study, we newly designed a fully automated cell culture system for human iPS maintenance. Using an automated culture system, hiPS cells maintained their undifferentiated state for 60 days. Automatically prepared hiPS cells had a potency of differentiation into three germ layer cells including dopaminergic neurons and pancreatic cells.
|
| Volume |
5
|
| Pages |
16647
|
| Published |
2015-11-17
|
| DOI |
10.1038/srep16647
|
| PII |
srep16647
|
| PMID |
26573336
|
| PMC |
PMC4647834
|
| MeSH |
Automation
Cell Culture Techniques / instrumentation
Cell Culture Techniques / methods*
Cell Differentiation
Cells, Cultured
Flow Cytometry
Humans
Immunohistochemistry
Induced Pluripotent Stem Cells / cytology*
Induced Pluripotent Stem Cells / metabolism
Karyotyping
Transcription Factors / genetics
Transcription Factors / metabolism
|
| IF |
3.998
|
| Times Cited |
28
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
454E2(HPS0077)
HiPS-RIKEN-2A(HPS0009) |
