論文 - 詳細
| RRC ID | 45503 |
|---|---|
| 著者 | Taniguchi T, Yamazaki W, Saeki Y, Takajo I, Okayama A, Hayashi T, Misawa N. |
| タイトル | The pathogenic potential of Helicobacter cinaedi isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells in vitro. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Helicobacter cinaedi infection has been recognized as an increasingly important emerging disease in humans. Infection with H. cinaedi causes bacteremia, cellulitis and enteritis. H. cinaedi has been isolated from non-human sources, including dogs, cats and rodents; however, it remains unclear whether animal strains are pathogenic in humans and as zoonotic pathogens. In this study, H. cinaedi isolates were recovered from a dog and a hamster, and the ability of these isolates to adhere to, invade and translocate across polarized human intestinal epithelial Caco-2 cells was examined in vitro. To better understand the pathogenic potential of animal H. cinaedi isolates, these results were compared with those for a human strain that was isolated from a patient with bacteremia. The animal and human strains adhered to and invaded Caco-2 cells, but to a lesser degree than the C. jejuni 81-176 strain, which was used as a control. The integrity of tight junctions was monitored by measuring transepithelial electrical resistance (TER) with a membrane insert system. The TER values for all H. cinaedi strains did not change during the experimental periods compared with those of the controls; however, translocation of H. cinaedi from the apical side to the basolateral side was confirmed by cultivation and H. cinaedi-specific PCR, suggesting that the H. cinaedi strains translocated by transcellular route. This study demonstrated that H. cinaedi strains of animal origin might have a pathogenic potential in human epithelial cells as observed in a translocation assay in vitro with a human isolate. |
| 巻・号 | 78(4) |
| ページ | 627-32 |
| 公開日 | 2016-5-3 |
| DOI | 10.1292/jvms.15-0595 |
| PMID | 26685883 |
| PMC | PMC4873854 |
| MeSH | Animals Bacterial Adhesion Caco-2 Cells Cell Polarity Cricetinae Dogs Helicobacter / isolation & purification Helicobacter / pathogenicity* Humans Intestinal Mucosa / microbiology* |
| IF | 1.049 |
| 引用数 | 5 |
| WOS 分野 | VETERINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |