RRC ID 45525
Author Makino A, Abe M, Ishitsuka R, Murate M, Kishimoto T, Sakai S, Hullin-Matsuda F, Shimada Y, Inaba T, Miyatake H, Tanaka H, Kurahashi A, Pack CG, Kasai RS, Kubo S, Schieber NL, Dohmae N, Tochio N, Hagiwara K, Sasaki Y, Aida Y, Fujimori F, Kigawa T, Nishibori K, Parton RG, Kusumi A, Sako Y, Anderluh G, Yamashita M, Kobayashi T, Greimel P, Kobayashi T.
Title A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.
Journal FASEB J.
Abstract We identified a novel, nontoxic mushroom protein that specifically binds to a complex of sphingomyelin (SM), a major sphingolipid in mammalian cells, and cholesterol (Chol). The purified protein, termed nakanori, labeled cell surface domains in an SM- and Chol-dependent manner and decorated specific lipid domains that colocalized with inner leaflet small GTPase H-Ras, but not K-Ras. The use of nakanori as a lipid-domain-specific probe revealed altered distribution and dynamics of SM/Chol on the cell surface of Niemann-Pick type C fibroblasts, possibly explaining some of the disease phenotype. In addition, that nakanori treatment of epithelial cells after influenza virus infection potently inhibited virus release demonstrates the therapeutic value of targeting specific lipid domains for anti-viral treatment.-Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.-G., Kasai, R. S., Kubo, S., Schieber, N. L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton, R. G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T. A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C.
Volume 31(4)
Pages 1301-1322
Published 2017-4
DOI 10.1096/fj.201500075R
PII fj.201500075R
PMID 27492925
MeSH Binding Sites Cells, Cultured Cholesterol / metabolism* Fibroblasts / drug effects Fibroblasts / metabolism Fungal Proteins / chemistry Fungal Proteins / metabolism Fungal Proteins / pharmacology* Grifola / chemistry* HeLa Cells Humans Membrane Microdomains / drug effects* Membrane Microdomains / metabolism Membrane Microdomains / virology Niemann-Pick Disease, Type C / metabolism* Protein Binding Sphingomyelins / metabolism* Virus Release
IF 5.595
Times Cited 0
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY BIOLOGY CELL BIOLOGY
Resource
DNA material pET28/His6-EGFP-Nakanori (RDB14301) pET28/His6-mCherry-D4 (RDB14300).