RRC ID 45531
Author Bohr T, Nelson CR, Klee E, Bhalla N.
Title Spindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans.
Journal J. Cell Biol.
Abstract Homologue synapsis is required for meiotic chromosome segregation, but how synapsis is initiated between chromosomes is poorly understood. In Caenorhabditis elegans, synapsis and a checkpoint that monitors synapsis depend on pairing centers (PCs), cis-acting loci that interact with nuclear envelope proteins, such as SUN-1, to access cytoplasmic microtubules. Here, we report that spindle assembly checkpoint (SAC) components MAD-1, MAD-2, and BUB-3 are required to negatively regulate synapsis and promote the synapsis checkpoint response. Both of these roles are independent of a conserved component of the anaphase-promoting complex, indicating a unique role for these proteins in meiotic prophase. MAD-1 and MAD-2 localize to the periphery of meiotic nuclei and interact with SUN-1, suggesting a role at PCs. Consistent with this idea, MAD-1 and BUB-3 require full PC function to inhibit synapsis. We propose that SAC proteins monitor the stability of pairing, or tension, between homologues to regulate synapsis and elicit a checkpoint response.
Volume 211(2)
Pages 233-42
Published 2015-10-26
DOI 10.1083/jcb.201409035
PII jcb.201409035
PMID 26483555
PMC PMC4621841
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Cell Nucleus / metabolism Chromosome Pairing* Chromosome Segregation Chromosomes / metabolism Dyneins / genetics Dyneins / metabolism Mad2 Proteins / genetics Mad2 Proteins / metabolism* Meiosis* Nuclear Proteins / genetics Nuclear Proteins / metabolism* RNA Interference RNA, Small Interfering Receptors, Cytoplasmic and Nuclear / metabolism Spindle Apparatus / metabolism*
IF 8.784
Times Cited 3
C.elegans tm1458 tm3039 tm2190