Reference - Detail
| RRC ID | 45538 |
|---|---|
| Author | Cheong MC, Artyukhin AB, You YJ, Avery L. |
| Title | An opioid-like system regulating feeding behavior in C. elegans. |
| Journal | Elife |
| Abstract |
Neuropeptides are essential for the regulation of appetite. Here we show that neuropeptides could regulate feeding in mutants that lack neurotransmission from the motor neurons that stimulate feeding muscles. We identified nlp-24 by an RNAi screen of 115 neuropeptide genes, testing whether they affected growth. NLP-24 peptides have a conserved YGGXX sequence, similar to mammalian opioid neuropeptides. In addition, morphine and naloxone respectively stimulated and inhibited feeding in starved worms, but not in worms lacking NPR-17, which encodes a protein with sequence similarity to opioid receptors. Opioid agonists activated heterologously expressed NPR-17, as did at least one NLP-24 peptide. Worms lacking the ASI neurons, which express npr-17, did not response to naloxone. Thus, we suggest that Caenorhabditis elegans has an endogenous opioid system that acts through NPR-17, and that opioids regulate feeding via ASI neurons. Together, these results suggest C. elegans may be the first genetically tractable invertebrate opioid model. |
| Volume | 4 |
| Published | 2015-4-21 |
| DOI | 10.7554/eLife.06683 |
| PMID | 25898004 |
| PMC | PMC4427864 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / drug effects Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism Conserved Sequence Feeding Behavior / drug effects Feeding Behavior / physiology* Gene Expression Regulation Molecular Sequence Data Morphine / pharmacology Naloxone / pharmacology Neurons / cytology Neurons / drug effects Neurons / metabolism* Neuropeptides / genetics Neuropeptides / metabolism* Receptors, Opioid / deficiency Receptors, Opioid / genetics* Signal Transduction Starvation / metabolism |
| IF | 7.08 |
| Times Cited | 20 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | News |
| Total number of mentions | 14 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3210 tm3225 tm3023 tm2105 |