RRC ID 45544
Author Cohen ML, Kim S, Morita K, Kim SH, Han M.
Title The GATA factor elt-1 regulates C. elegans developmental timing by promoting expression of the let-7 family microRNAs.
Journal PLoS Genet.
Abstract Postembryonic development in Caenorhabditis elegans is a powerful model for the study of the temporal regulation of development and for the roles of microRNAs in controlling gene expression. Stable switch-like changes in gene expression occur during development as stage-specific microRNAs are expressed and subsequently down-regulate other stage-specific factors, driving developmental progression. Key genes in this regulatory network are phylogenetically conserved and include the post-transcriptional microRNA repressor LIN-28; the nuclear hormone receptor DAF-12; and the microRNAs LIN-4, LET-7, and the three LET-7 family miRNAs (miR-48, miR-84, and miR-241). DAF-12 is known to regulate transcription of miR-48, miR-84 and miR-241, but its contribution is insufficient to account for all of the transcriptional regulation implied by the mutant phenotypes. In this work, the GATA-family transcription factor ELT-1 is identified from a genetic enhancer screen as a regulator of developmental timing in parallel to DAF-12, and is shown to do so by promoting the expression of the LET-7, miR-48, miR-84, and miR-241 microRNAs. The role of ELT-1 in developmental timing is shown to be separate from its role in cell-fate maintenance during post-embryonic development. In addition, analysis of Chromatin Immnoprecipitation (ChIP) data from the modENCODE project and this work suggest that the contribution of ELT-1 to the control of let-7 family microRNA expression is likely through direct transcription regulation.
Volume 11(3)
Pages e1005099
Published 2015-3
DOI 10.1371/journal.pgen.1005099
PMID 25816370
PMC PMC4376641
MeSH Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / biosynthesis* Caenorhabditis elegans Proteins / genetics Cell Differentiation / genetics* GATA Transcription Factors / biosynthesis* GATA Transcription Factors / genetics Gene Expression Regulation, Developmental MicroRNAs / biosynthesis* MicroRNAs / genetics Mutation Receptors, Cytoplasmic and Nuclear / genetics Transcription, Genetic*
IF 5.54
Times Cited 5
C.elegans tm4063