RRC ID 45551
著者 De Henau S, Tilleman L, Vangheel M, Luyckx E, Trashin S, Pauwels M, Germani F, Vlaeminck C, Vanfleteren JR, Bert W, Pesce A, Nardini M, Bolognesi M, De Wael K, Moens L, Dewilde S, Braeckman BP.
タイトル A redox signalling globin is essential for reproduction in Caenorhabditis elegans.
ジャーナル Nat Commun
Abstract Moderate levels of reactive oxygen species (ROS) are now recognized as redox signalling molecules. However, thus far, only mitochondria and NADPH oxidases have been identified as cellular sources of ROS in signalling. Here we identify a globin (GLB-12) that produces superoxide, a type of ROS, which serves as an essential signal for reproduction in C. elegans. We find that GLB-12 has an important role in the regulation of multiple aspects in germline development, including germ cell apoptosis. We further describe how GLB-12 displays specific molecular, biochemical and structural properties that allow this globin to act as a superoxide generator. In addition, both an intra- and extracellular superoxide dismutase act as key partners of GLB-12 to create a transmembrane redox signal. Our results show that a globin can function as a driving factor in redox signalling, and how this signal is regulated at the subcellular level by multiple control layers.
巻・号 6
ページ 8782
公開日 2015-12-1
DOI 10.1038/ncomms9782
PII ncomms9782
PMID 26621324
PMC PMC4686822
MeSH Animals Caenorhabditis elegans / chemistry Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Germ Cells / metabolism Globins / chemistry Globins / genetics Globins / metabolism* Models, Molecular Reproduction Signal Transduction Superoxides / metabolism*
IF 12.121
引用数 21
WOS 分野 BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
線虫 tm776 tm760 tm1146 tm536