RRC ID 45560
Author Fukuyama M, Kontani K, Katada T, Rougvie AE.
Title The C. elegans Hypodermis Couples Progenitor Cell Quiescence to the Dietary State.
Journal Curr Biol
Abstract The nutritional status of an organism can greatly impact the function and behavior of stem and progenitor cells [1]. However, the regulatory circuits that inform these cells about the dietary environment remain to be elucidated. Newly hatched C. elegans larvae (L1s) halt development in "L1 arrest" or "L1 diapause" until ample food is encountered and triggers stem and progenitor cells to exit from quiescence [2]. The insulin/insulin-like growth factor signaling (IIS) pathway plays a key role in this reactivation [3, 4], but its site(s) of action have not been elucidated nor have the nutrient molecule(s) that stimulate the pathway been identified. By tissue-specifically modulating the activity of its components, we demonstrate that the IIS pathway acts in the hypodermis to regulate nutrition-responsive reactivation of neural and mesodermal progenitor cells. We identify ethanol, a likely component of the natural Caenorhabditis habitat, and amino acids as nutrients that synergistically reactivate somatic progenitor cells and upregulate expression of insulin-like genes in starved L1 larvae. The hypodermis likely senses the availability of amino acids because forced activation of the amino-acid-responsive Rag-TORC1 (target of rapamycin complex 1) pathway in this tissue can also release somatic progenitor cell quiescence in the presence of ethanol. Finally, there appears to be crosstalk between the IIS and Rag-TORC1 pathways because constitutive activation of the IIS pathway requires Rag to promote reactivation. This work demonstrates that ethanol and amino acids act as dietary cues via the IIS and Rag-TORC1 pathways in the hypodermis to coordinately control progenitor cell behavior.
Volume 25(9)
Pages 1241-8
Published 2015-5-4
DOI 10.1016/j.cub.2015.03.016
PII S0960-9822(15)00331-0
PMID 25891400
MeSH Amino Acids / pharmacology* Animals Caenorhabditis elegans / drug effects* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism Cell Movement Diet Ethanol / pharmacology* Proto-Oncogene Proteins c-akt / metabolism Receptor, Insulin / metabolism Stem Cells / drug effects* Stem Cells / metabolism Subcutaneous Tissue / metabolism
IF 9.601
Times Cited 18
WOS Category BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
Resource
C.elegans tm2983 tm2988 tm2560 tm1974 tm2308