論文 - 詳細
| RRC ID | 45600 |
|---|---|
| 著者 | Luz AL, Rooney JP, Kubik LL, Gonzalez CP, Song DH, Meyer JN. |
| タイトル | Mitochondrial Morphology and Fundamental Parameters of the Mitochondrial Respiratory Chain Are Altered in Caenorhabditis elegans Strains Deficient in Mitochondrial Dynamics and Homeostasis Processes. |
| ジャーナル | PLoS One |
| Abstract |
Mitochondrial dysfunction has been linked to myriad human diseases and toxicant exposures, highlighting the need for assays capable of rapidly assessing mitochondrial health in vivo. Here, using the Seahorse XFe24 Analyzer and the pharmacological inhibitors dicyclohexylcarbodiimide and oligomycin (ATP-synthase inhibitors), carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (mitochondrial uncoupler) and sodium azide (cytochrome c oxidase inhibitor), we measured the fundamental parameters of mitochondrial respiratory chain function: basal oxygen consumption, ATP-linked respiration, maximal respiratory capacity, spare respiratory capacity and proton leak in the model organism Caenhorhabditis elegans. Since mutations in mitochondrial homeostasis genes cause mitochondrial dysfunction and have been linked to human disease, we measured mitochondrial respiratory function in mitochondrial fission (drp-1)-, fusion (fzo-1)-, mitophagy (pdr-1, pink-1)-, and electron transport chain complex III (isp-1)-deficient C. elegans. All showed altered function, but the nature of the alterations varied between the tested strains. We report increased basal oxygen consumption in drp-1; reduced maximal respiration in drp-1, fzo-1, and isp-1; reduced spare respiratory capacity in drp-1 and fzo-1; reduced proton leak in fzo-1 and isp-1; and increased proton leak in pink-1 nematodes. As mitochondrial morphology can play a role in mitochondrial energetics, we also quantified the mitochondrial aspect ratio for each mutant strain using a novel method, and for the first time report increased aspect ratios in pdr-1- and pink-1-deficient nematodes. |
| 巻・号 | 10(6) |
| ページ | e0130940 |
| 公開日 | 2015-1-1 |
| DOI | 10.1371/journal.pone.0130940 |
| PII | PONE-D-15-03794 |
| PMID | 26106885 |
| PMC | PMC4480853 |
| MeSH | 2,4-Dinitrophenol / pharmacology Adenosine Triphosphate / metabolism Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology Dicyclohexylcarbodiimide / pharmacology Dynamins / deficiency Dynamins / genetics Electron Transport* Electron Transport Complex III / deficiency Electron Transport Complex III / genetics GTP Phosphohydrolases / deficiency GTP Phosphohydrolases / genetics Homeostasis Mitochondria / drug effects Mitochondria / metabolism Mitochondria / ultrastructure* Mitochondria, Muscle / drug effects Mitochondria, Muscle / metabolism Mitochondria, Muscle / ultrastructure Oligomycins / pharmacology Oxygen Consumption Protein Serine-Threonine Kinases / deficiency Protein Serine-Threonine Kinases / genetics Sodium Azide / pharmacology Ubiquitin-Protein Ligases / deficiency Ubiquitin-Protein Ligases / genetics |
| IF | 2.74 |
| 引用数 | 33 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1133 tm1779 tm1108 |