論文 - 詳細
| RRC ID | 45651 |
|---|---|
| 著者 | Vandamme J, Sidoli S, Mariani L, Friis C, Christensen J, Helin K, Jensen ON, Salcini AE. |
| タイトル | H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans. |
| ジャーナル | Nucleic Acids Res |
| Abstract |
Genome-wide analyses in Caenorhabditis elegans show that post-translational modifications (PTMs) of histones are evolutionary conserved and distributed along functionally distinct genomic domains. However, a global profile of PTMs and their co-occurrence on the same histone tail has not been described in this organism. We used mass spectrometry based middle-down proteomics to analyze histone H3 N-terminal tails from C. elegans embryos for the presence, the relative abundance and the potential cross-talk of co-existing PTMs. This analysis highlighted that the lysine 23 of histone H3 (H3K23) is extensively modified by methylation and that tri-methylated H3K9 (H3K9me3) is exclusively detected on histone tails with di-methylated H3K23 (H3K23me2). Chromatin immunoprecipitation approaches revealed a positive correlation between H3K23me2 and repressive marks. By immunofluorescence analyses, H3K23me2 appears differentially regulated in germ and somatic cells, in part by the action of the histone demethylase JMJD-1.2. H3K23me2 is enriched in heterochromatic regions, localizing in H3K9me3 and heterochromatin protein like-1 (HPL-1)-positive foci. Biochemical analyses indicated that HPL-1 binds to H3K23me2 and interacts with a conserved CoREST repressive complex. Thus, our study suggests that H3K23me2 defines repressive domains and contributes to organizing the genome in distinct heterochromatic regions during embryogenesis. |
| 巻・号 | 43(20) |
| ページ | 9694-710 |
| 公開日 | 2015-11-16 |
| DOI | 10.1093/nar/gkv1063 |
| PII | gkv1063 |
| PMID | 26476455 |
| PMC | PMC4787770 |
| MeSH | Acetylation Animals Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / analysis Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Chromosomal Proteins, Non-Histone / analysis Co-Repressor Proteins / metabolism Embryo, Nonmammalian / metabolism Embryonic Development Germ Cells / metabolism Heterochromatin / metabolism* Histone Code* Histone Demethylases / metabolism Histones / analysis Histones / chemistry Histones / metabolism* Lysine / metabolism Methylation Protein Processing, Post-Translational |
| IF | 11.502 |
| 引用数 | 18 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3713 tm1489 tm4586 |