論文 - 詳細
| RRC ID | 45671 |
|---|---|
| 著者 | Ackema KB, Hench J, Böckler S, Wang SC, Sauder U, Mergentaler H, Westermann B, Bard F, Frank S, Spang A. |
| タイトル | The small GTPase Arf1 modulates mitochondrial morphology and function. |
| ジャーナル | EMBO J |
| Abstract |
The small GTPase Arf1 plays critical roles in membrane traffic by initiating the recruitment of coat proteins and by modulating the activity of lipid-modifying enzymes. Here, we report an unexpected but evolutionarily conserved role for Arf1 and the ArfGEF GBF1 at mitochondria. Loss of function of ARF-1 or GBF-1 impaired mitochondrial morphology and activity in Caenorhabditis elegans. Similarly, mitochondrial defects were observed in mammalian and yeast cells. In Saccharomyces cerevisiae, aberrant clusters of the mitofusin Fzo1 accumulated in arf1-11 mutants and were resolved by overexpression of Cdc48, an AAA-ATPase involved in ER and mitochondria-associated degradation processes. Yeast Arf1 co-fractionated with ER and mitochondrial membranes and interacted genetically with the contact site component Gem1. Furthermore, similar mitochondrial abnormalities resulted from knockdown of either GBF-1 or contact site components in worms, suggesting that the role of Arf1 in mitochondrial functioning is linked to ER-mitochondrial contacts. Thus, Arf1 is involved in mitochondrial homeostasis and dynamics, independent of its role in vesicular traffic. |
| 巻・号 | 33(22) |
| ページ | 2659-75 |
| 公開日 | 2014-11-18 |
| DOI | 10.15252/embj.201489039 |
| PII | embj.201489039 |
| PMID | 25190516 |
| PMC | PMC4282574 |
| MeSH | ADP-Ribosylation Factor 1 / genetics ADP-Ribosylation Factor 1 / metabolism* Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* GTP Phosphohydrolases / genetics GTP Phosphohydrolases / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Mitochondria / enzymology* Mitochondria / genetics Mitochondrial Membranes / enzymology Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Saccharomyces cerevisiae / enzymology* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae Proteins / genetics Saccharomyces cerevisiae Proteins / metabolism |
| IF | 9.889 |
| 引用数 | 38 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1108 tm1133 |