論文 - 詳細
| RRC ID | 45751 |
|---|---|
| 著者 | Hughes S, Wilkinson H, Gilbert SP, Kishida M, Ding SS, Woollard A. |
| タイトル | The C. elegans TPR Containing Protein, TRD-1, Regulates Cell Fate Choice in the Developing Germ Line and Epidermis. |
| ジャーナル | PLoS One |
| Abstract |
Correct cell fate choice is crucial in development. In post-embryonic development of the hermaphroditic Caenorhabitis elegans, distinct cell fates must be adopted in two diverse tissues. In the germline, stem cells adopt one of three possible fates: mitotic cell cycle, or gamete formation via meiosis, producing either sperm or oocytes. In the epidermis, the stem cell-like seam cells divide asymmetrically, with the daughters taking on either a proliferative (seam) or differentiated (hypodermal or neuronal) fate. We have isolated a novel conserved C. elegans tetratricopeptide repeat containing protein, TRD-1, which is essential for cell fate determination in both the germline and the developing epidermis and has homologs in other species, including humans (TTC27). We show that trd-1(RNAi) and mutant animals have fewer seam cells as a result of inappropriate differentiation towards the hypodermal fate. In the germline, trd-1 RNAi results in a strong masculinization phenotype, as well as defects in the mitosis to meiosis switch. Our data suggests that trd-1 acts downstream of tra-2 but upstream of fem-3 in the germline sex determination pathway, and exhibits a constellation of phenotypes in common with other Mog (masculinization of germline) mutants. Thus, trd-1 is a new player in both the somatic and germline cell fate determination machinery, suggestive of a novel molecular connection between the development of these two diverse tissues. |
| 巻・号 | 9(12) |
| ページ | e114998 |
| 公開日 | 2014-1-1 |
| DOI | 10.1371/journal.pone.0114998 |
| PII | PONE-D-14-43364 |
| PMID | 25493563 |
| PMC | PMC4262444 |
| MeSH | Animals Blotting, Western Caenorhabditis elegans / growth & development* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / physiology* Cell Cycle Proteins / physiology* Cell Differentiation / physiology* Cell Lineage / physiology Epidermis / growth & development* Female Germ Cells / growth & development* Germ Cells / physiology Male Meiosis / physiology Mitosis / physiology Sex Determination Processes / physiology |
| IF | 2.74 |
| 引用数 | 3 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2764 |