論文 - 詳細
| RRC ID | 45778 |
|---|---|
| 著者 | Kuzmanov A, Yochem J, Fay DS. |
| タイトル | Analysis of PHA-1 reveals a limited role in pharyngeal development and novel functions in other tissues. |
| ジャーナル | Genetics |
| Abstract |
PHA-1 encodes a cytoplasmic protein that is required for embryonic morphogenesis and attachment of the foregut (pharynx) to the mouth (buccal capsule). Previous reports have in some cases suggested that PHA-1 is essential for the differentiation of most or all pharyngeal cell types. By performing mosaic analysis with a recently acquired pha-1 null mutation (tm3671), we found that PHA-1 is not required within most or all pharyngeal cells for their proper specification, differentiation, or function. Rather, our evidence suggests that PHA-1 acts in the arcade or anterior epithelial cells of the pharynx to promote attachment of the pharynx to the future buccal capsule. In addition, PHA-1 appears to be required in the epidermis for embryonic morphogenesis, in the excretory system for osmoregulation, and in the somatic gonad for normal ovulation and fertility. PHA-1 activity is also required within at least a subset of intestinal cells for viability. To better understand the role of PHA-1 in the epidermis, we analyzed several apical junction markers in pha-1(tm3671) homozygous embryos. PHA-1 regulates the expression of several components of two apical junction complexes including AJM-1-DLG-1/discs large complex and the classical cadherin-catenin complex, which may account for the role of PHA-1 in embryonic morphogenesis. |
| 巻・号 | 198(1) |
| ページ | 259-68 |
| 公開日 | 2014-9-1 |
| DOI | 10.1534/genetics.114.166876 |
| PII | genetics.114.166876 |
| PMID | 25009149 |
| PMC | PMC4174937 |
| MeSH | Animals Cadherins / genetics Cadherins / metabolism Caenorhabditis elegans / embryology Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Catenins / genetics Catenins / metabolism Cell Differentiation Epithelial Cells / cytology Epithelial Cells / metabolism Guanylate Kinases / genetics Guanylate Kinases / metabolism Organ Specificity Pharynx / cytology Pharynx / embryology Pharynx / metabolism* Tight Junctions / metabolism |
| IF | 4.015 |
| 引用数 | 4 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3671 |