RRC ID 45809
著者 Morales-Martínez A, Dobrzynska A, Askjaer P.
タイトル Inner nuclear membrane protein LEM-2 is required for correct nuclear separation and morphology in C. elegans.
ジャーナル J Cell Sci
Abstract The inner nuclear membrane proteins emerin and LEMD2 have both overlapping and separate functions in regulation of nuclear organization, gene expression and cell differentiation. We report here that emerin (EMR-1) and LEM domain protein 2 (LEM-2) are expressed in all tissues throughout Caenorhaditis elegans development but their relative distribution differs between cell types. The ratio of EMR-1 to LEM-2 is particularly high in contractile tissues, intermediate in neurons and hypodermis and lowest in intestine and germ line. We find that LEM-2 is recruited earlier than EMR-1 to reforming nuclear envelopes, suggesting the presence of separate mitotic membrane compartments and specific functions of each protein. Concordantly, we observe that nuclei of lem-2 mutant embryos, but not of emr-1 mutants, have reduced nuclear circularity. Finally, we uncover a so-far-unknown role of LEM-2 in nuclear separation and anchoring of microtubule organizing centers.
巻・号 128(6)
ページ 1090-6
公開日 2015-3-15
DOI 10.1242/jcs.164202
PII jcs.164202
PMID 25653391
MeSH Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / growth & development Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins Cell Nucleus / physiology* Cell Nucleus / ultrastructure* Fluorescent Antibody Technique Image Processing, Computer-Assisted Membrane Proteins / genetics Membrane Proteins / metabolism* Nuclear Envelope / physiology* Nuclear Proteins / genetics Nuclear Proteins / metabolism*
IF 4.573
引用数 12
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1582