| RRC ID |
45812
|
| Author |
Nagy AI, Vázquez-Manrique RP, Lopez M, Christov CP, Sequedo MD, Herzog M, Herlihy AE, Bodak M, Gatsi R, Baylis HA.
|
| Title |
IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans.
|
| Journal |
EMBO Rep
|
| Abstract |
RNA interference (RNAi) is a widespread and widely exploited phenomenon. Here, we show that changing inositol 1,4,5-trisphosphate (IP3) signalling alters RNAi sensitivity in Caenorhabditis elegans. Reducing IP3 signalling enhances sensitivity to RNAi in a broad range of genes and tissues. Conversely up-regulating IP3 signalling decreases sensitivity. Tissue-specific rescue experiments suggest IP3 functions in the intestine. We also exploit IP3 signalling mutants to further enhance the sensitivity of RNAi hypersensitive strains. These results demonstrate that conserved cell signalling pathways can modify RNAi responses, implying that RNAi responses may be influenced by an animal's physiology or environment.
|
| Volume |
16(3)
|
| Pages |
341-50
|
| Published |
2015-3-1
|
| DOI |
10.15252/embr.201439585
|
| PII |
embr.201439585
|
| PMID |
25608529
|
| PMC |
PMC4353841
|
| MeSH |
Animals
Caenorhabditis elegans / genetics
Caenorhabditis elegans / physiology*
Image Processing, Computer-Assisted
Inositol 1,4,5-Trisphosphate / metabolism*
Intestinal Mucosa / metabolism
Microscopy, Fluorescence
Models, Biological
RNA Interference / physiology*
RNA, Double-Stranded
Signal Transduction / genetics
Signal Transduction / physiology*
|
| IF |
7.497
|
| Times Cited |
1
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm902
tm753 |
