論文 - 詳細
| RRC ID | 45815 |
|---|---|
| 著者 | Neumann B, Coakley S, Giordano-Santini R, Linton C, Lee ES, Nakagawa A, Xue D, Hilliard MA. |
| タイトル | EFF-1-mediated regenerative axonal fusion requires components of the apoptotic pathway. |
| ジャーナル | Nature |
| Abstract |
Functional regeneration after nervous system injury requires transected axons to reconnect with their original target tissue. Axonal fusion, a spontaneous regenerative mechanism identified in several species, provides an efficient means of achieving target reconnection as a regrowing axon is able to contact and fuse with its own separated axon fragment, thereby re-establishing the original axonal tract. Here we report a molecular characterization of this process in Caenorhabditis elegans, revealing dynamic changes in the subcellular localization of the EFF-1 fusogen after axotomy, and establishing phosphatidylserine (PS) and the PS receptor (PSR-1) as critical components for axonal fusion. PSR-1 functions cell-autonomously in the regrowing neuron and, instead of acting in its canonical signalling pathway, acts in a parallel phagocytic pathway that includes the transthyretin protein TTR-52, as well as CED-7, NRF-5 and CED-6 (refs 9, 10, 11, 12). We show that TTR-52 binds to PS exposed on the injured axon, and can restore fusion several hours after injury. We propose that PS functions as a 'save-me' signal for the distal fragment, allowing conserved apoptotic cell clearance molecules to function in re-establishing axonal integrity during regeneration of the nervous system. |
| 巻・号 | 517(7533) |
| ページ | 219-22 |
| 公開日 | 2015-1-8 |
| DOI | 10.1038/nature14102 |
| PII | nature14102 |
| PMID | 25567286 |
| MeSH | ATP-Binding Cassette Transporters / metabolism Animals Apoptosis / physiology* Apoptosis Regulatory Proteins Axons / metabolism* Axons / pathology Caenorhabditis elegans / cytology* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / metabolism Growth Cones / metabolism Membrane Glycoproteins / metabolism* Mutation Nerve Regeneration / physiology* Phagocytes / metabolism Phagocytosis Phosphatidylserines / metabolism Phosphoproteins / metabolism Receptors, Cell Surface / metabolism Signal Transduction Spectrin / genetics Spectrin / metabolism |
| IF | 42.779 |
| 引用数 | 59 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 38 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2078 tm469 |