論文 - 詳細
| RRC ID | 45837 |
|---|---|
| 著者 | Reid A, Yücel D, Wood M, Llamosas E, Kant S, Crossley M, Nicholas H. |
| タイトル | The transcriptional repressor CTBP-1 functions in the nervous system of Caenorhabditis elegans to regulate lifespan. |
| ジャーナル | Exp Gerontol |
| Abstract |
C-terminal binding proteins (CtBPs) are recruited by a variety of transcription factors to mediate gene repression. Nematode CTBP-1 has previously been shown to play a role in the regulation of lifespan; Caenorhabditis elegans strains carrying a deletion in the ctbp-1 gene showed a 10-20% increase in mean and maximal lifespan compared with wild-type control strains. We set out to identify the tissues in which CTBP-1 functions to regulate lifespan in C. elegans. Our analysis of reporter genes shows that CTBP-1 is predominantly expressed in the nervous system with lower levels detectable in the hypodermis. Tissue-specific rescue experiments demonstrated that CTBP-1 functions in the nervous system to regulate lifespan. Previously, the lifespan extension in a ctbp-1 mutant was attributed, at least in part, to the misregulation of a lipase gene, lips-7. We therefore focussed on lips-7 and found that expressing CTBP-1 solely in the nervous system of a ctbp-1 mutant significantly reduced lips-7 transcription. In addition, we studied another ctbp-1 mutant allele that also displayed a long-lived phenotype. In this case, lips-7 expression was unaffected. This observation argues that, while lips-7 may play a role in lifespan, its de-repression is not essential for the extension of lifespan phenotype. We show that a prominent site of LIPS-7 expression is the hypodermis, one of the sites of fat storage in C. elegans. Interestingly, we did not observe co-localisation of CTBP-1 and lips-7 transcription in the nervous system, indicating that CTBP-1 may be acting indirectly, in a cell non-autonomous manner. In summary, our data confirm that CTBP-1 is involved in the regulation of lips-7 transcription but suggest that it may perform additional roles in the nervous system that contribute to the regulation of longevity. |
| 巻・号 | 60 |
| ページ | 153-65 |
| 公開日 | 2014-12-1 |
| DOI | 10.1016/j.exger.2014.09.022 |
| PII | S0531-5565(14)00286-1 |
| PMID | 25456848 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / physiology* Gene Expression Regulation Genes, Helminth Genes, Reporter Lipase / genetics Longevity / genetics Longevity / physiology* Mutation Nervous System Physiological Phenomena Protein Isoforms / genetics Protein Isoforms / physiology Repressor Proteins / genetics Repressor Proteins / physiology* |
| IF | 3.376 |
| 引用数 | 3 |
| WOS 分野 | GERIATRICS & GERONTOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | NA |