RRC ID 4585
Author Koito A, Kameyama Y, Cheng-Mayer C, Matsushita S.
Title Susceptibility of mink (Mustera vision)-derived cells to replication by human immunodeficiency virus type 1.
Journal J Virol
Abstract In vivo studies for understanding viral transmission and replication, host immune responses, and pathogenesis of human immunodeficiency virus type 1 (HIV-1) infection would greatly benefit from the establishment of a small-animal model. In this study, we explored the potential of American mink (Mustera vison) as a susceptible host. We found that primary cells and cell lines derived from this species efficiently supported trans-activation of the HIV-1 long terminal repeat by Tat. Accordingly, the cysteine residue at position 261, which has been shown to be important for interaction of the human cyclin T1 with the HIV-1 regulatory protein Tat, is conserved in the mink homologue. No species-specific defect in Rev function could be detected in mink cells. In addition, primary splenocytes, fibroblasts, and the Mv.1.Lu cell line from American mink supported early as well as late HIV-1 gene expression following infection with vesicular stomatitis G protein-pseudotyped HIV-1 viruses, at levels comparable to those seen with permissive human cells. Furthermore, the mink Mv.1.Lu cell line stably expressing human CD4 and CCR5 receptors supported a spreading HIV-1 infection with few, if any, deficiencies compared to findings in human cell lines. This indicates the potential of HIV-1 to replicate in these cells once the blockade at the stage of virus entry has been removed. These results clearly show that cells from American mink generally pose no functional intracellular block to HIV-1 replication, and collectively they raise the possibility that this animal species could be engineered to support HIV-1 infection, providing a useful small-animal model for evaluating de novo infection by HIV-1.
Volume 77(9)
Pages 5109-17
Published 2003-5-1
DOI 10.1128/jvi.77.9.5109-5117.2003
PMID 12692213
PMC PMC153984
MeSH Amino Acid Sequence Animals Cell Line Cells, Cultured Cyclin T Cyclins / metabolism Disease Models, Animal* Fibroblasts / cytology Fibroblasts / virology* Gene Expression Gene Products, tat / metabolism HIV Infections / virology HIV Long Terminal Repeat / genetics HIV-1 / genetics HIV-1 / physiology* Humans Mice Mink / virology* Molecular Sequence Data Spleen / cytology Spleen / virology* Virus Replication* tat Gene Products, Human Immunodeficiency Virus
IF 4.501
Times Cited 9
Human and Animal Cells Mv.1.Lu(NBL-7)(RCB0996)