論文 - 詳細
| RRC ID | 45857 |
|---|---|
| 著者 | Simon M, Sarkies P, Ikegami K, Doebley AL, Goldstein LD, Mitchell J, Sakaguchi A, Miska EA, Ahmed S. |
| タイトル | Reduced insulin/IGF-1 signaling restores germ cell immortality to Caenorhabditis elegans Piwi mutants. |
| ジャーナル | Cell Rep |
| Abstract |
Defects in the Piwi/piRNA pathway lead to transposon desilencing and immediate sterility in many organisms. We found that the C. elegans Piwi mutant prg-1 became sterile after growth for many generations. This phenotype did not occur for RNAi mutants with strong transposon-silencing defects and was separable from the role of PRG-1 in transgene silencing. Brief periods of starvation extended the transgenerational lifespan of prg-1 mutants by stimulating the DAF-16/FOXO longevity transcription factor. Constitutive activation of DAF-16 via reduced daf-2 insulin/IGF-1 signaling immortalized prg-1 strains via RNAi proteins and histone H3 lysine 4 demethylases. In late-generation prg-1 mutants, desilencing of repetitive segments of the genome occurred, and silencing of repetitive loci was restored in prg-1; daf-2 mutants. This study reveals an unexpected interface between aging and transgenerational maintenance of germ cells, where somatic longevity is coupled to a genome-silencing pathway that promotes germ cell immortality in parallel to the Piwi/piRNA system. |
| 巻・号 | 7(3) |
| ページ | 762-73 |
| 公開日 | 2014-5-8 |
| DOI | 10.1016/j.celrep.2014.03.056 |
| PII | S2211-1247(14)00253-8 |
| PMID | 24767993 |
| PMC | PMC4049074 |
| MeSH | Animals Argonaute Proteins / antagonists & inhibitors Argonaute Proteins / genetics* Argonaute Proteins / metabolism Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / antagonists & inhibitors Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Forkhead Transcription Factors Germ Cells / cytology Germ Cells / metabolism* Insulin / metabolism* Insulin-Like Growth Factor I / metabolism* Oxidoreductases, N-Demethylating / metabolism RNA Interference RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Receptor, Insulin / deficiency Receptor, Insulin / genetics Receptor, Insulin / metabolism Signal Transduction Transcription Factors / metabolism |
| IF | 8.109 |
| 引用数 | 45 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm872 |