論文 - 詳細
| RRC ID | 45864 |
|---|---|
| 著者 | Tavernier N, Noatynska A, Panbianco C, Martino L, Van Hove L, Schwager F, Léger T, Gotta M, Pintard L. |
| タイトル | Cdk1 phosphorylates SPAT-1/Bora to trigger PLK-1 activation and drive mitotic entry in C. elegans embryos. |
| ジャーナル | J Cell Biol |
| Abstract |
The molecular mechanisms governing mitotic entry during animal development are incompletely understood. Here, we show that the mitotic kinase CDK-1 phosphorylates Suppressor of Par-Two 1 (SPAT-1)/Bora to regulate its interaction with PLK-1 and to trigger mitotic entry in early Caenorhabditis elegans embryos. Embryos expressing a SPAT-1 version that is nonphosphorylatable by CDK-1 and that is defective in PLK-1 binding in vitro present delays in mitotic entry, mimicking embryos lacking SPAT-1 or PLK-1 functions. We further show that phospho-SPAT-1 activates PLK-1 by triggering phosphorylation on its activator T loop in vitro by Aurora A. Likewise, we show that phosphorylation of human Bora by Cdk1 promotes phosphorylation of human Plk1 by Aurora A, suggesting that this mechanism is conserved in humans. Our results suggest that CDK-1 activates PLK-1 via SPAT-1 phosphorylation to promote entry into mitosis. We propose the existence of a positive feedback loop that connects Cdk1 and Plk1 activation to ensure a robust control of mitotic entry and cell division timing. |
| 巻・号 | 208(6) |
| ページ | 661-9 |
| 公開日 | 2015-3-16 |
| DOI | 10.1083/jcb.201408064 |
| PII | jcb.201408064 |
| PMID | 25753036 |
| PMC | PMC4362466 |
| MeSH | Amino Acid Sequence Animals Aurora Kinase A / metabolism CDC2 Protein Kinase / physiology* Caenorhabditis elegans / cytology* Caenorhabditis elegans / enzymology Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / metabolism* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Enzyme Activation Humans Larva / cytology Larva / enzymology Mitosis Molecular Sequence Data Phosphorylation Protein Processing, Post-Translational Protein Serine-Threonine Kinases / metabolism* Sf9 Cells Spodoptera |
| IF | 8.811 |
| 引用数 | 23 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm3061 tm3060 |