RRC ID 45887
Author Wu Q, Zhao Y, Li Y, Wang D.
Title Molecular signals regulating translocation and toxicity of graphene oxide in the nematode Caenorhabditis elegans.
Journal Nanoscale
Abstract Both in vitro and in vivo studies have demonstrated the toxic effects of graphene oxide (GO). However, the molecular basis for the translocation and toxicity of GO is still largely unclear. In the present study, we employed an in vivo Caenorhabditis elegans assay system to identify molecular signals involved in the control of the translocation and toxicity of GO. We identified 7 genes whose mutations altered both the translocation and toxicity of GO. Mutations of the hsp-16.48, gas-1, sod-2, sod-3, and aak-2 genes caused greater GO translocation into the body and toxic effects on both primary and secondary targeted organs compared with wild type; however, mutations of the isp-1 and clk-1 genes resulted in significantly decreased GO translocation into the body and toxicity on both primary and secondary targeted organs compared with wild-type. Moreover, mutations of the hsp-16.48, gas-1, sod-2, sod-3, and aak-2 genes caused increased intestinal permeability and prolonged mean defecation cycle length in GO-exposed nematodes, whereas mutations of the isp-1 and clk-1 genes resulted in decreased intestinal permeability in GO-exposed nematodes. Therefore, for the underlying mechanism, we hypothesize that both intestinal permeability and defecation behavior may have crucial roles in controlling the functions of the identified molecular signals. The molecular signals may further contribute to the control of transgenerational toxic effects of GO. Our results provide an important insight into understanding the molecular basis for the in vivo translocation and toxicity of GO.
Volume 6(19)
Pages 11204-12
Published 2014-10-7
DOI 10.1039/c4nr02688h
PMID 25124895
MeSH Animals Caenorhabditis elegans / drug effects* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / metabolism* Graphite / toxicity* Materials Testing Nanoparticles / toxicity* Organ Specificity Oxides / toxicity Signal Transduction / drug effects* Signal Transduction / physiology Tissue Distribution
IF 7.233
Times Cited 19
WOS Category PHYSICS, APPLIED MATERIALS SCIENCE, MULTIDISCIPLINARY CHEMISTRY, MULTIDISCIPLINARY NANOSCIENCE & NANOTECHNOLOGY
Resource
C.elegans tm1770 tm776 tm1146