RRC ID 45903
Author Zheng S, Liao S, Zou Y, Qu Z, Shen W, Shi Y.
Title Mulberry leaf polyphenols delay aging and regulate fat metabolism via the germline signaling pathway in Caenorhabditis elegans.
Journal Age (Dordr)
Abstract Mulberry leaves are an important ingredient in some traditional Chinese medicinal formulas and has been developed for use in functional food products. The antioxidant activity of mulberry leaf extract has been reported to have beneficial effects on diseases in vitro; however, it is not clear which components in mulberry leaf extracts have these functions. Furthermore, the mechanisms of action of these ingredients have not been extensively investigated. In this study, we extracted total mulberry leaf polyphenols (MLP) and identified its 13 phenolic monomers. Our results, using Caenorhabditis elegans as a model, indicated that MLPs delayed aging, improved oxidative stress resistance, and reduced fatty acid storage in vivo. Subsequent genetic screens and gene expression analyses demonstrated that the functions of MLP mainly depended on the germline signaling pathway, thus influencing the activities of downstream transcription factors (DAF-12, DAF-16, PHA-4, and NHR-80) as well as the expression levels of their target genes (fat-6, lipl-4, sod-3, unc-51, and fard-1). Our study determined that diverse modes of action on longevity were promoted by MLP exposure. These observations provide the first insight into MLP's multifaceted functions on aging, fat accumulation, and reproduction in vivo and indicate a specific model for the mechanism of action of MLP. This is a significant finding that lends support to the hypotheses that mulberry leaf extracts can have an impact on human health.
Volume 36(6)
Pages 9719
Published 2014-1-1
DOI 10.1007/s11357-014-9719-z
PMID 25323576
PMC PMC4199944
MeSH Adipose Tissue / drug effects Adipose Tissue / metabolism* Aging / drug effects* Aging / genetics Animals Caenorhabditis elegans / drug effects* Caenorhabditis elegans / genetics Disease Models, Animal Germ-Line Mutation Morus Plant Extracts / pharmacology* Plant Leaves Polyphenols / pharmacology* Random Allocation Sensitivity and Specificity Signal Transduction
IF 4.648
Times Cited 20
C.elegans tm1011