論文 - 詳細
| RRC ID | 45931 |
|---|---|
| 著者 | Bowman EA, Bowman CR, Ahn JH, Kelly WG. |
| タイトル | Phosphorylation of RNA polymerase II is independent of P-TEFb in the C. elegans germline. |
| ジャーナル | Development |
| Abstract |
RNA polymerase II (Pol II) elongation in metazoans is thought to require phosphorylation of serine 2 (Ser2-P) of the Pol II C-terminal domain (CTD) by the P-TEFb complex, CDK-9/cyclin T. Another Ser2 kinase complex, CDK-12/cyclin K, which requires upstream CDK-9 activity has been identified in Drosophila and human cells. We show that regulation of Ser2-P in C. elegans soma is similar to other metazoan systems, but Ser2-P in the germline is independent of CDK-9, and largely requires only CDK-12. The observed differences are not due to differential tissue expression as both kinases and their cyclin partners are ubiquitously expressed. Surprisingly, loss of CDK-9 from germ cells has little effect on Ser2-P, yet CDK-9 is essential for germline development. By contrast, loss of CDK-12 and Ser2-P specifically from germ cells has little impact on germline development or function, although significant loss of co-transcriptional H3K36 trimethylation is observed. These results show a reduced requirement for Pol II Ser2-P in germline development and suggest that generating Ser2-P is not the essential role of CDK-9 in these cells. Transcriptional elongation in the C. elegans germline thus appears to be uniquely regulated, which may be a novel facet of germline identity. |
| 巻・号 | 140(17) |
| ページ | 3703-13 |
| 公開日 | 2013-9-1 |
| DOI | 10.1242/dev.095778 |
| PII | dev.095778 |
| PMID | 23903194 |
| PMC | PMC3742150 |
| MeSH | Animals Animals, Genetically Modified Blotting, Western Caenorhabditis elegans / enzymology* Clutch Size Cyclin-Dependent Kinases / metabolism Germ Cells / metabolism* Microscopy, Fluorescence Phosphorylation Positive Transcriptional Elongation Factor B / metabolism* RNA Interference RNA Polymerase II / metabolism* Real-Time Polymerase Chain Reaction Serine / metabolism |
| IF | 5.611 |
| 引用数 | 20 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2884 tm3846 |