RRC ID 45953
著者 Clemons AM, Brockway HM, Yin Y, Kasinathan B, Butterfield YS, Jones SJ, Colaiácovo MP, Smolikove S.
タイトル akirin is required for diakinesis bivalent structure and synaptonemal complex disassembly at meiotic prophase I.
ジャーナル Mol Biol Cell
Abstract During meiosis, evolutionarily conserved mechanisms regulate chromosome remodeling, leading to the formation of a tight bivalent structure. This bivalent, a linked pair of homologous chromosomes, is essential for proper chromosome segregation in meiosis. The formation of a tight bivalent involves chromosome condensation and restructuring around the crossover. The synaptonemal complex (SC), which mediates homologous chromosome association before crossover formation, disassembles concurrently with increased condensation during bivalent remodeling. Both chromosome condensation and SC disassembly are likely critical steps in acquiring functional bivalent structure. The mechanisms controlling SC disassembly, however, remain unclear. Here we identify akir-1 as a gene involved in key events of meiotic prophase I in Caenorhabditis elegans. AKIR-1 is a protein conserved among metazoans that lacks any previously known function in meiosis. We show that akir-1 mutants exhibit severe meiotic defects in late prophase I, including improper disassembly of the SC and aberrant chromosome condensation, independently of the condensin complexes. These late-prophase defects then lead to aberrant reconfiguring of the bivalent. The meiotic divisions are delayed in akir-1 mutants and are accompanied by lagging chromosomes. Our analysis therefore provides evidence for an important role of proper SC disassembly in configuring a functional bivalent structure.
巻・号 24(7)
ページ 1053-67
公開日 2013-4-1
DOI 10.1091/mbc.E12-11-0841
PII mbc.E12-11-0841
PMID 23363597
PMC PMC3608493
MeSH Alleles Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Cycle Proteins / genetics Cell Cycle Proteins / metabolism* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism Chromosome Pairing Chromosomes / genetics Chromosomes / metabolism Crossing Over, Genetic Female In Situ Hybridization, Fluorescence Luminescent Proteins / genetics Luminescent Proteins / metabolism Male Meiotic Prophase I* Microscopy, Fluorescence Mutation Nuclear Proteins / genetics Nuclear Proteins / metabolism Oocytes / cytology Oocytes / metabolism RNA Interference Rad51 Recombinase / genetics Rad51 Recombinase / metabolism Synaptonemal Complex / metabolism* Time-Lapse Imaging
IF 3.791
引用数 20
WOS 分野 CELL BIOLOGY
リソース情報
線虫 tm1833