論文 - 詳細
| RRC ID | 4597 |
|---|---|
| 著者 | Hirata N, Takahashi M, Yazawa M. |
| タイトル | Diphosphorylation of regulatory light chain of myosin IIA is responsible for proper cell spreading. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The actomyosin cytoskeleton plays prominent roles in cell spreading and migration. To address the roles of myosin II isoforms and to estimate the region where the myosin IIs are activated in spreading cells, we examined the immunolocalization of myosin II isoforms and phosphorylated RLCs in the spreading MRC-5 cells. We observed the formation of actin ring-like structure at the base of the lamella. Both myosin IIA and IIB were predominantly localized there. Myosin IIA and diphosphorylated RLC were distributed outside of the region where myosin IIB and monophosphoryated RLC were distributed predominantly. Inhibition of Rho-kinase resulted in the disappearance of the diphosphorylation of RLC, moreover, it accelerated the rate of cell spreading and induced an aberrant cell shape at later stage of spreading. These results indicate that diphosphorylation of RLCs of myosin IIA by Rho-kinase in lamella is responsible for the cell to spread properly. |
| 巻・号 | 381(4) |
| ページ | 682-7 |
| 公開日 | 2009-4-17 |
| DOI | 10.1016/j.bbrc.2009.02.121 |
| PII | S0006-291X(09)00400-8 |
| PMID | 19254691 |
| MeSH | Cell Movement* / drug effects Humans Myosin Light Chains / metabolism* Nonmuscle Myosin Type IIA / metabolism* Nonmuscle Myosin Type IIB / metabolism Phosphorylation / drug effects Protein Kinase Inhibitors / pharmacology rho-Associated Kinases / metabolism |
| IF | 2.985 |
| 引用数 | 15 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5 |