Reference - Detail
| RRC ID | 46014 |
|---|---|
| Author | Kovacevic I, Orozco JM, Cram EJ. |
| Title | Filamin and phospholipase C-ε are required for calcium signaling in the Caenorhabditis elegans spermatheca. |
| Journal | PLoS Genet |
| Abstract |
The Caenorhabditis elegans spermatheca is a myoepithelial tube that stores sperm and undergoes cycles of stretching and constriction as oocytes enter, are fertilized, and exit into the uterus. FLN-1/filamin, a stretch-sensitive structural and signaling scaffold, and PLC-1/phospholipase C-ε, an enzyme that generates the second messenger IP3, are required for embryos to exit normally after fertilization. Using GCaMP, a genetically encoded calcium indicator, we show that entry of an oocyte into the spermatheca initiates a distinctive series of IP3-dependent calcium oscillations that propagate across the tissue via gap junctions and lead to constriction of the spermatheca. PLC-1 is required for the calcium release mechanism triggered by oocyte entry, and FLN-1 is required for timely initiation of the calcium oscillations. INX-12, a gap junction subunit, coordinates propagation of the calcium transients across the spermatheca. Gain-of-function mutations in ITR-1/IP3R, an IP3-dependent calcium channel, and loss-of-function mutations in LFE-2, a negative regulator of IP3 signaling, increase calcium release and suppress the exit defect in filamin-deficient animals. We further demonstrate that a regulatory cassette consisting of MEL-11/myosin phosphatase and NMY-1/non-muscle myosin is required for coordinated contraction of the spermatheca. In summary, this study answers long-standing questions concerning calcium signaling dynamics in the C. elegans spermatheca and suggests FLN-1 is needed in response to oocyte entry to trigger calcium release and coordinated contraction of the spermathecal tissue. |
| Volume | 9(5) |
| Pages | e1003510 |
| Published | 2013-5-1 |
| DOI | 10.1371/journal.pgen.1003510 |
| PII | PGENETICS-D-12-02663 |
| PMID | 23671426 |
| PMC | PMC3650001 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Calcium / metabolism* Calcium Signaling / genetics* Calcium Signaling / physiology Contractile Proteins / genetics* Contractile Proteins / metabolism Female Filamins Inositol 1,4,5-Trisphosphate / genetics Inositol 1,4,5-Trisphosphate / metabolism Male Microfilament Proteins / genetics* Microfilament Proteins / metabolism Oocytes / metabolism Oocytes / physiology Phosphoinositide Phospholipase C / genetics* Phosphoinositide Phospholipase C / metabolism Spermatozoa / metabolism Spermatozoa / physiology |
| IF | 5.175 |
| Times Cited | 25 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm545 |