論文 - 詳細
| RRC ID | 46039 |
|---|---|
| 著者 | Large EE, Mathies LD. |
| タイトル | Caenorhabditis elegans SWI/SNF subunits control sequential developmental stages in the somatic gonad. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
The Caenorhabditis elegans somatic gonadal precursors (SGPs) are multipotent progenitors that give rise to all somatic tissues of the adult reproductive system. The hunchback and Ikaros-like gene ehn-3 is expressed specifically in SGPs and is required for their development into differentiated tissues of the somatic gonad. To find novel genes involved in SGP development, we used a weak allele of ehn-3 as the basis for a reverse genetic screen. Feeding RNAi was used to screen ∼2400 clones consisting of transcription factors, signaling components, and chromatin factors. The screen identified five members of the C. elegans SWI/SNF chromatin remodeling complex as genetic enhancers of ehn-3. We characterized alleles of 10 SWI/SNF genes and found that SWI/SNF subunits are required for viability and gonadogenesis. Two conserved SWI/SNF complexes, PBAF and BAF, are defined by their unique array of accessory subunits around a common enzymatic core that includes a catalytic Swi2/Snf2-type ATPase. Tissue-specific RNAi experiments suggest that C. elegans PBAF and BAF complexes control different processes during somatic gonadal development: PBRM-1, a signature subunit of PBAF, is important for normal SGP development, whereas LET-526, the distinguishing subunit of BAF, is required for development of a differentiated cell type, the distal tip cell (DTC). We found that the SWSN-4 ATPase subunit is required for SGP and DTC development. Finally, we provide evidence that C. elegans PBAF subunits and hnd-1/dHand are important for the cell fate decision between SGPs and their differentiated sisters, the head mesodermal cells. |
| 巻・号 | 4(3) |
| ページ | 471-83 |
| 公開日 | 2014-3-20 |
| DOI | 10.1534/g3.113.009852 |
| PII | g3.113.009852 |
| PMID | 24402584 |
| PMC | PMC3962486 |
| MeSH | Adenosine Triphosphatases / genetics Adenosine Triphosphatases / metabolism Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosomal Proteins, Non-Histone / genetics Chromosomal Proteins, Non-Histone / metabolism DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism Gonads / growth & development* Mesoderm / metabolism Phenotype RNA Interference Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 2.781 |
| 引用数 | 7 |
| WOS 分野 | GENETICS & HEREDITY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm415 tm4795 tm3395 tm4263 tm3309 tm3647 tm305 tm4567 tm2127 |