| RRC ID |
46063
|
| Author |
Lin L, Yang P, Huang X, Zhang H, Lu Q, Zhang H.
|
| Title |
The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic assembly machinery.
|
| Journal |
J Cell Biol
|
| Abstract |
The mechanism by which protein aggregates are selectively degraded by autophagy is poorly understood. Previous studies show that a family of Atg8-interacting proteins function as receptors linking specific cargoes to the autophagic machinery. Here we demonstrate that during Caenorhabditis elegans embryogenesis, epg-7 functions as a scaffold protein mediating autophagic degradation of several protein aggregates, including aggregates of the p62 homologue SQST-1, but has little effect on other autophagy-regulated processes. EPG-7 self-oligomerizes and is degraded by autophagy independently of SQST-1. SQST-1 directly interacts with EPG-7 and colocalizes with EPG-7 aggregates in autophagy mutants. Mutations in epg-7 impair association of SQST-1 aggregates with LGG-1/Atg8 puncta. EPG-7 interacts with multiple ATG proteins and colocalizes with ATG-9 puncta in various autophagy mutants. Unlike core autophagy genes, epg-7 is dispensable for starvation-induced autophagic degradation of substrate aggregates. Our results indicate that under physiological conditions a scaffold protein endows cargo specificity and also elevates degradation efficiency by linking the cargo-receptor complex with the autophagic machinery.
|
| Volume |
201(1)
|
| Pages |
113-29
|
| Published |
2013-4-1
|
| DOI |
10.1083/jcb.201209098
|
| PII |
jcb.201209098
|
| PMID |
23530068
|
| PMC |
PMC3613692
|
| MeSH |
Animals
Autophagy / physiology*
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology*
Embryonic Development / physiology*
Mutation
Protein Multimerization / physiology*
|
| IF |
8.811
|
| Times Cited |
45
|
|
WOS Category
|
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm2508
tm3425 |
