Reference - Detail
| RRC ID | 46063 |
|---|---|
| Author | Lin L, Yang P, Huang X, Zhang H, Lu Q, Zhang H. |
| Title | The scaffold protein EPG-7 links cargo-receptor complexes with the autophagic assembly machinery. |
| Journal | J Cell Biol |
| Abstract |
The mechanism by which protein aggregates are selectively degraded by autophagy is poorly understood. Previous studies show that a family of Atg8-interacting proteins function as receptors linking specific cargoes to the autophagic machinery. Here we demonstrate that during Caenorhabditis elegans embryogenesis, epg-7 functions as a scaffold protein mediating autophagic degradation of several protein aggregates, including aggregates of the p62 homologue SQST-1, but has little effect on other autophagy-regulated processes. EPG-7 self-oligomerizes and is degraded by autophagy independently of SQST-1. SQST-1 directly interacts with EPG-7 and colocalizes with EPG-7 aggregates in autophagy mutants. Mutations in epg-7 impair association of SQST-1 aggregates with LGG-1/Atg8 puncta. EPG-7 interacts with multiple ATG proteins and colocalizes with ATG-9 puncta in various autophagy mutants. Unlike core autophagy genes, epg-7 is dispensable for starvation-induced autophagic degradation of substrate aggregates. Our results indicate that under physiological conditions a scaffold protein endows cargo specificity and also elevates degradation efficiency by linking the cargo-receptor complex with the autophagic machinery. |
| Volume | 201(1) |
| Pages | 113-29 |
| Published | 2013-4-1 |
| DOI | 10.1083/jcb.201209098 |
| PII | jcb.201209098 |
| PMID | 23530068 |
| PMC | PMC3613692 |
| MeSH | Animals Autophagy / physiology* Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / embryology* Embryonic Development / physiology* Mutation Protein Multimerization / physiology* |
| IF | 8.811 |
| Times Cited | 45 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Clinical guideline |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2508 tm3425 |