Reference - Detail
| RRC ID | 46071 |
|---|---|
| Author | Maekawa M, Terasaka S, Mochizuki Y, Kawai K, Ikeda Y, Araki N, Skolnik EY, Taguchi T, Arai H. |
| Title | Sequential breakdown of 3-phosphorylated phosphoinositides is essential for the completion of macropinocytosis. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
Macropinocytosis is a highly conserved endocytic process by which extracellular fluid and solutes are internalized into cells. Macropinocytosis starts with the formation of membrane ruffles at the plasma membrane and ends with their closure. The transient and sequential emergence of phosphoinositides PI(3,4,5)P3 and PI(3,4)P2 in the membrane ruffles is essential for macropinocytosis. By making use of information in the Caenorhabditis elegans mutants defective in fluid-phase endocytosis, we found that mammalian phosphoinositide phosphatase MTMR6 that dephosphorylates PI(3)P to PI, and its binding partner MTMR9, are required for macropinocytosis. INPP4B, which dephosphorylates PI(3,4)P2 to PI(3)P, was also found to be essential for macropinocytosis. These phosphatases operate after the formation of membrane ruffles to complete macropinocytosis. Finally, we showed that KCa3.1, a Ca(2+)-activated K(+) channel that is activated by PI(3)P, is required for macropinocytosis. We propose that the sequential breakdown of PI(3,4,5)P3 → PI(3,4)P2 → PI(3)P → PI controls macropinocytosis through specific effectors of the intermediate phosphoinositides. |
| Volume | 111(11) |
| Pages | E978-87 |
| Published | 2014-3-18 |
| DOI | 10.1073/pnas.1311029111 |
| PII | 1311029111 |
| PMID | 24591580 |
| PMC | PMC3964036 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans / physiology* Cell Line DNA Primers / genetics Humans Microscopy, Electron, Scanning Microscopy, Fluorescence Phosphatidylinositol Phosphates / metabolism* Phosphoric Monoester Hydrolases / metabolism* Phosphorylation Pinocytosis / physiology* Protein Tyrosine Phosphatases, Non-Receptor / metabolism* RNA Interference RNA, Small Interfering / genetics Reverse Transcriptase Polymerase Chain Reaction |
| IF | 9.412 |
| Times Cited | 40 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1125 tm3733 tm2036 tm246 |