論文 - 詳細
| RRC ID | 46072 |
|---|---|
| 著者 | Edmonds JW, McKinney SL, Prasain JK, Miller MA. |
| タイトル | The gap junctional protein INX-14 functions in oocyte precursors to promote C. elegans sperm guidance. |
| ジャーナル | Dev Biol |
| Abstract |
Innexins are the subunits of invertebrate gap junctions. Here we show that the innexin INX-14 promotes sperm guidance to the fertilization site in the Caenorhabditis elegans hermaphrodite reproductive tract. inx-14 loss causes cell nonautonomous defects in sperm migration velocity and directional velocity. Results from genetic and immunocytochemical analyses provide strong evidence that INX-14 acts in transcriptionally active oocyte precursors in the distal gonad, not in transcriptionally inactive oocytes that synthesize prostaglandin sperm-attracting cues. Somatic gonadal sheath cell interaction is necessary for INX-14 function, likely via INX-8 and INX-9 expressed in sheath cells. However, electron microscopy has not identified gap junctions in oocyte precursors, suggesting that INX-14 acts in a channel-independent manner or INX-14 channels are difficult to document. INX-14 promotes prostaglandin signaling to sperm at a step after F-series prostaglandin synthesis in oocytes. Taken together, our results support the model that INX-14 functions in a somatic gonad/germ cell signaling mechanism essential for sperm function. We propose that this mechanism regulates the transcription of a factor(s) that modulates prostaglandin metabolism, transport, or activity in the reproductive tract. |
| 巻・号 | 359(1) |
| ページ | 47-58 |
| 公開日 | 2011-11-1 |
| DOI | 10.1016/j.ydbio.2011.08.014 |
| PII | S0012-1606(11)01201-2 |
| PMID | 21889935 |
| PMC | PMC3192278 |
| MeSH | Animals Caenorhabditis elegans / cytology Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / physiology* Chromatography, Liquid Fatty Acids / administration & dosage Female Gap Junctions / metabolism* Male Microinjections Prostaglandins / metabolism RNA Interference Signal Transduction Spectrometry, Mass, Electrospray Ionization Spermatozoa / cytology* Tandem Mass Spectrometry Transcription Factors / metabolism |
| IF | 2.896 |
| 引用数 | 14 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1661 |