Reference - Detail
| RRC ID | 46075 |
|---|---|
| Author | Elle IC, Simonsen KT, Olsen LC, Birck PK, Ehmsen S, Tuck S, Le TT, Færgeman NJ. |
| Title | Tissue- and paralogue-specific functions of acyl-CoA-binding proteins in lipid metabolism in Caenorhabditis elegans. |
| Journal | Biochem J |
| Abstract |
ACBP (acyl-CoA-binding protein) is a small primarily cytosolic protein that binds acyl-CoA esters with high specificity and affinity. ACBP has been identified in all eukaryotic species, indicating that it performs a basal cellular function. However, differential tissue expression and the existence of several ACBP paralogues in many eukaryotic species indicate that these proteins serve distinct functions. The nematode Caenorhabditis elegans expresses seven ACBPs: four basal forms and three ACBP domain proteins. We find that each of these paralogues is capable of complementing the growth of ACBP-deficient yeast cells, and that they exhibit distinct temporal and tissue expression patterns in C. elegans. We have obtained loss-of-function mutants for six of these forms. All single mutants display relatively subtle phenotypes; however, we find that functional loss of ACBP-1 leads to reduced triacylglycerol (triglyceride) levels and aberrant lipid droplet morphology and number in the intestine. We also show that worms lacking ACBP-2 show a severe decrease in the β-oxidation of unsaturated fatty acids. A quadruple mutant, lacking all basal ACBPs, is slightly developmentally delayed, displays abnormal intestinal lipid storage, and increased β-oxidation. Collectively, the present results suggest that each of the ACBP paralogues serves a distinct function in C. elegans. |
| Volume | 437(2) |
| Pages | 231-41 |
| Published | 2011-7-15 |
| DOI | 10.1042/BJ20102099 |
| PII | BJ20102099 |
| PMID | 21539519 |
| MeSH | Amino Acid Sequence Animals Animals, Genetically Modified Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / physiology Carrier Proteins / genetics Carrier Proteins / metabolism Diazepam Binding Inhibitor / metabolism* Fatty Acids, Unsaturated / metabolism Lipid Metabolism Molecular Sequence Data Mutation Protein Isoforms / genetics* Protein Isoforms / metabolism Receptors, Cytoplasmic and Nuclear / physiology Starvation / metabolism Transcription Factors / physiology Triglycerides / metabolism |
| IF | 4.097 |
| Times Cited | 27 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3573 tm2896 tm2995 |