Reference - Detail
| RRC ID | 46092 |
|---|---|
| Author | Murayama T, Takayama J, Fujiwara M, Maruyama IN. |
| Title | Environmental alkalinity sensing mediated by the transmembrane guanylyl cyclase GCY-14 in C. elegans. |
| Journal | Curr Biol |
| Abstract |
Survival requires that living organisms continuously monitor environmental and tissue pH. Animals sense acidic pH using ion channels and G-protein-coupled receptors (GPCRs), but monitoring of alkaline pH is not well understood. We report here that in the nematode Caenorhabditis elegans, a transmembrane receptor-type guanylyl cyclase (RGC), GCY-14, of the ASEL gustatory neuron, plays an essential role in the sensing of extracellular alkalinity. Activation of GCY-14 opens a cGMP-gated cation channel encoded by tax-2 and tax-4, resulting in Ca(2+) entry into ASEL. Ectopic expression of GCY-14 in other neurons indicates that it accounts for the alkalinity sensing capability. Domain-swapping and site-directed mutagenesis of GCY-14 reveal that GCY-14 functions as a homodimer, in which histidine of the extracellular domains plays a crucial role in alkalinity detection. These results argue that in addition to ion channels and GPCRs, RGCs also play a role in pH sensation in neurons. |
| Volume | 23(11) |
| Pages | 1007-12 |
| Published | 2013-6-3 |
| DOI | 10.1016/j.cub.2013.04.052 |
| PII | S0960-9822(13)00493-4 |
| PMID | 23664973 |
| MeSH | Animals Caenorhabditis elegans / genetics* Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / genetics* Caenorhabditis elegans Proteins / metabolism Calcium Signaling* Cyclic GMP / metabolism* Guanylate Cyclase / genetics* Guanylate Cyclase / metabolism Hydrogen-Ion Concentration Ion Channels / metabolism Molecular Sequence Data Neurons / metabolism Neurons / physiology |
| IF | 9.601 |
| Times Cited | 18 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm3098 tm1449 tm901 tm2669 tm1653 tm897 tm2364 |