論文 - 詳細
| RRC ID | 46111 |
|---|---|
| 著者 | Hayes GD, Riedel CG, Ruvkun G. |
| タイトル | The Caenorhabditis elegans SOMI-1 zinc finger protein and SWI/SNF promote regulation of development by the mir-84 microRNA. |
| ジャーナル | Genes Dev |
| Abstract |
Hundreds of microRNAs (miRNAs) have been discovered in metazoans and plants, and understanding of their biogenesis has advanced dramatically; however, relatively little is known about the cofactors necessary for miRNA regulation of target gene expression. In Caenorhabditis elegans, the conserved miRNA let-7 and its paralogs, including mir-84, control the timing of stage-specific developmental events. To identify factors required for the activity of mir-84 and possibly other miRNAs, we screened for mutations that suppress the developmental defects caused by overexpression of mir-84. Mutations in the somi-1 gene prevent these defects without affecting the expression level of mir-84. Loss of somi-1 also causes phenotypes similar to deletion of mir-84, showing that somi-1 is necessary for the normal function of this miRNA. somi-1 encodes a zinc finger protein that localizes to nuclear foci and binds the promoters of let-60/RAS, lin-14, and lin-28, genes that may be targeted by mir-84 and similar miRNAs. Genetic evidence shows that somi-1 inhibits lin-14 and induction of the vulval precursors by the let-60/RAS pathway. Proteomic and genetic screens identified conserved chromatin-remodeling and homeodomain transcription factor complexes that work with somi-1 to regulate differentiation. Our results suggest that somi-1 coordinates a nuclear response that complements the activity of mir-84. |
| 巻・号 | 25(19) |
| ページ | 2079-92 |
| 公開日 | 2011-10-1 |
| DOI | 10.1101/gad.17153811 |
| PII | 25/19/2079 |
| PMID | 21979920 |
| PMC | PMC3197206 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Differentiation Cell Nucleus / metabolism DNA Helicases / chemistry DNA Helicases / metabolism* DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Gene Expression Regulation, Developmental MicroRNAs / metabolism* Molecular Sequence Data Promoter Regions, Genetic Protein Binding Protein Transport Sequence Alignment Subcutaneous Tissue / growth & development |
| IF | 9.527 |
| 引用数 | 11 |
| WOS 分野 | GENETICS & HEREDITY DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm562 tm1304 |