RRC ID |
46113
|
著者 |
Nix P, Hammarlund M, Hauth L, Lachnit M, Jorgensen EM, Bastiani M.
|
タイトル |
Axon regeneration genes identified by RNAi screening in C. elegans.
|
ジャーナル |
J Neurosci
|
Abstract |
Axons of the mammalian CNS lose the ability to regenerate soon after development due to both an inhibitory CNS environment and the loss of cell-intrinsic factors necessary for regeneration. The complex molecular events required for robust regeneration of mature neurons are not fully understood, particularly in vivo. To identify genes affecting axon regeneration in Caenorhabditis elegans, we performed both an RNAi-based screen for defective motor axon regeneration in unc-70/β-spectrin mutants and a candidate gene screen. From these screens, we identified at least 50 conserved genes with growth-promoting or growth-inhibiting functions. Through our analysis of mutants, we shed new light on certain aspects of regeneration, including the role of β-spectrin and membrane dynamics, the antagonistic activity of MAP kinase signaling pathways, and the role of stress in promoting axon regeneration. Many gene candidates had not previously been associated with axon regeneration and implicate new pathways of interest for therapeutic intervention.
|
巻・号 |
34(2)
|
ページ |
629-45
|
公開日 |
2014-1-8
|
DOI |
10.1523/JNEUROSCI.3859-13.2014
|
PII |
34/2/629
|
PMID |
24403161
|
PMC |
PMC3870940
|
MeSH |
Animals
Axons / physiology*
Caenorhabditis elegans / genetics*
Nerve Regeneration / genetics*
RNA, Small Interfering
Signal Transduction / physiology*
|
IF |
5.674
|
引用数 |
51
|
WOS 分野
|
NEUROSCIENCES
|
リソース情報 |
線虫 |
tm3821
tm3754
tm1991
tm1110
tm556
tm3464
tm2688
tm1238
tm4000
tm3359
tm2180
tm1711
tm2426
tm3686
tm648
tm734
tm2266
tm364
tm4028
tm3933
tm5591
tm5298
tm5280
tm5115 |