RRC ID 46113
著者 Nix P, Hammarlund M, Hauth L, Lachnit M, Jorgensen EM, Bastiani M.
タイトル Axon regeneration genes identified by RNAi screening in C. elegans.
ジャーナル J Neurosci
Abstract Axons of the mammalian CNS lose the ability to regenerate soon after development due to both an inhibitory CNS environment and the loss of cell-intrinsic factors necessary for regeneration. The complex molecular events required for robust regeneration of mature neurons are not fully understood, particularly in vivo. To identify genes affecting axon regeneration in Caenorhabditis elegans, we performed both an RNAi-based screen for defective motor axon regeneration in unc-70/β-spectrin mutants and a candidate gene screen. From these screens, we identified at least 50 conserved genes with growth-promoting or growth-inhibiting functions. Through our analysis of mutants, we shed new light on certain aspects of regeneration, including the role of β-spectrin and membrane dynamics, the antagonistic activity of MAP kinase signaling pathways, and the role of stress in promoting axon regeneration. Many gene candidates had not previously been associated with axon regeneration and implicate new pathways of interest for therapeutic intervention.
巻・号 34(2)
ページ 629-45
公開日 2014-1-8
DOI 10.1523/JNEUROSCI.3859-13.2014
PII 34/2/629
PMID 24403161
PMC PMC3870940
MeSH Animals Axons / physiology* Caenorhabditis elegans / genetics* Nerve Regeneration / genetics* RNA, Small Interfering Signal Transduction / physiology*
IF 5.674
引用数 51
WOS 分野 NEUROSCIENCES
リソース情報
線虫 tm3821 tm3754 tm1991 tm1110 tm556 tm3464 tm2688 tm1238 tm4000 tm3359 tm2180 tm1711 tm2426 tm3686 tm648 tm734 tm2266 tm364 tm4028 tm3933 tm5591 tm5298 tm5280 tm5115